高效液相色谱法测定新藤黄酸纳米粒中新藤黄酸的含量和包封率

目的：建立新藤黄酸纳米粒中新藤黄酸含量和包封率的测定方法。方法：采用C18-ODS色谱柱（250mm×4．6mm，5μm），流动相为甲醇：1．0mol／L磷酸（9：1），流速为1．0ml／min，检测波长为360nm，进样容积为20μl，柱温为25℃。结果：新藤黄酸在10～120μg范围内线性关系良好，r=0．9999（n=7）；新藤黄酸平均回收率为99．32％，RSD为1．03%（n=3）。平均含量为99．52％，包封率为83．81%.结论：高效液相色谱法准确、可靠、重复性好，可用于新藤黄酸纳米粒含量及其包封率的测定。

Determination of Content and Entrapment Efficiency of Neo-gambogic Acid Nanoparticles by HPLC

Objective： To establish a method for the determination of content and entrapment efficiency of neo-gambogie acid nanoparticles. Methods. Chromatographic conditions consisted of C18-columm, mobile phase （methanol： 1.0 mol/L H3PO4 ：9： 1）, LTV wavelength （360 nm）, flow rate （1.0 ml/min） and column temperature （25℃）. Results： Neo-gambogic acid showed a good linear relation with peak area at the range of 10-120μg , r ： 0. 999 9 （n：7）. The average recovery of neo-gambogic acid was 99.32%, RSD was 1.03% （n： 3）. The average content of neo-gambogic acid was 99.52%. Entrapment efficiency was 83.81%. Conclusion： This method possesses advantages of accuracy, reliability and good reproducibility, and it can be used for the determination of content and entrapment efficiency of neo-gambogic acid nanoparticles.