异甘草酸镁对CCl4致急性肝损伤大鼠肝组织CYP1A2、CYP2E1蛋白及其mRNA表达的影响

目的 观察异甘草酸镁对CCl4致急性肝损伤大鼠肝组织CYP1A2、CYP2E1蛋白及其mRNA表达的影响，从代谢酶方面探讨异甘草酸镁保肝作用的机制。方法 将大鼠分为正常对照组、模型组和给药组，每组6只。尾静脉注射给药，给药组给予20 mg/kg异甘草酸镁，正常组及模型组给予同容积生理盐水，连续14 d。模型组及给药组于给药第13天20：00点灌胃25% CCl4，剂量为1.6 mL/kg，制备大鼠急性肝损伤模型。各组大鼠于末次给药2 h后，从髂动脉放血处死，取肝组织进行苏木精-伊红染色，观察肝组织病理形态变化；采用RT-PCR及Western-blot检测各组大鼠肝组织中CYP1A2、CYP2E1 蛋白及mRNA表达水平。结果 肝组织病理学检查显示异甘草酸镁能改善肝组织损伤；模型组CYP1A2和CYP2E1蛋白及mRNA表达水平显著高于正常对照组（P<0.05），给药组CYP1A2和CYP2E1蛋白及mRNA表达水平较模型组显著降低（P<0.05）。结论 异甘草酸镁能够治疗肝损伤，其减毒机制可能与抑制CYP1A2、CYP2E1的表达有关。

Influence of Magnesium Isoglycyrrhizinate on Protein and mRNA Expression of CYP1A2 and CYP2E1 in Liver Tissues in Rats with Acute Liver Injury Induced by Carbon Tetrachloride

Objective To investigate the influence of magnesium isoglycyrrhizinate on the protein and mRNA expression of CYP1A2 and CYP2E1 in liver tissues in rats with acute liver injury induced by carbon tetrachloride (CCl4) and the mechanism of the liver-protecting effect of magnesium isoglycyrrhizinate from the aspect of metabolic enzymes. Methods Rats were randomly divided into normal control group, model group, and magnesium isoglycyrrhizinate group, with 6 rats in each group. All the rats were administered by caudal vein injection for 14 consecutive days; the rats in the magnesium isoglycyrrhizinate group were given magnesium isoglycyrrhizinate 20 mg/kg, and those in the normal control group and the model group were given normal saline of the same volume. The rats in the model group and the magnesium isoglycyrrhizinate group were given 25% CCl4 by gavage on day 13 at 20:00 at a dose of 1.6 mL/kg to establish a rat model of acute liver injury. At 2 hours after the last administration, the rats were sacrificed via exsanguination of the iliac artery, and the liver tissues were collected to observe pathomorphological changes of the liver by hematoxylin and eosin staining. RT-PCR and Western blot were used to measure the mRNA and protein expression of CYP1A2 and CYP2E1 in liver tissues. Results Liver pathology showed that magnesium isoglycyrrhizinate reduced liver injury. The model group had significantly higher protein and mRNA expression of CYP1A2 and CYP2E1 than the normal control group (P<0.05), and the magnesium isoglycyrrhizinate group had significantly lower protein and mRNA expression of CYP1A2 and CYP2E1 than the model group (P<0.05). Conclusion Magnesium isoglycyrrhizinate can reduce liver injury, and its attenuation mechanism may involve inhibiting the expression of CYP1A2 and CYP2E1.