DVC微量注射VIP对大鼠GMBF放大和电针调整效应

目的：采用中枢迷走神经背核复合体（DVC）微量注射血管活性肠肽（VIP），观察胃黏膜血流量（GMBF）变化和电针对GMBF的调整作用。方法：采用脑立体定位仪对大鼠中枢进行定位和注射，电针足三里穴，用氢气清除法测定GMBF；放射免疫法测量外周血中VIP含量。结果：DVC微量注射VIP后，血中VIP含量增加（P＜0．01），GMBF明显增加（P＜0．01）。电针（EA）足三里穴加强了GMBF放大效应。结论：DVC注射VIP对GMBF有放大效应，证实在神经－内分泌－免疫网络系统（NEIS）中VIP是重要的神经肽，DVC是VIP作用的社会中枢特异性部位之一，电针具有协同作用。

MAGNIFYING EFFECT OF MICRO-INJECTION OFVASOACTIVE INTESTINAL PEPTIDE INTO DORSAL MOTOR NUCLEUSOF VAGUS COMPLEX ON GASTRIC MUCOSA BLOOD FLOW ANDMODULATIONEFFECT OF ELECTRIC ACUPUNCTURE IN RATS

Objective:To investigate the changes of gastric mucosal blood flow(GMBF)and regulative effect of electro-acupuncture on GMBF in rats with micro-injection of vasoactive intestinal peptide(VIP) into dorsal motor nucleus of vagus complex(DVC).Method:The neurocentre of rat was located and injected by the stereotaxic apparatus of the rat brain,electro-acupuncture (EA)of Zusanli(ST-36) points was applied.GMBF was measured by the method of hydric gas clearance;The contents of VIP in the serum was assayed by radioimmunoassay.Result:The contents of VIP in the serum,transmucosal potential difference(PD) and GMBF were all increased(P<0.01) after micro-injection of VIP into DVC.These effects were enhanced by EA at Zusanli(ST-36) points.Conclusion:The magnifying effect on GMBF is induced by micro-injection of VIP into DVC,VIP is a strong neuropeptide in nerve endocrine immunity system(NEIS),DVC is a special part of neurocentre influenced by VIP,EA has the synergistic effects on micro-injection of VIP into DVC.