原发性高血压中医证型与血管紧张素原 M235T基因多态性相关性

目的探讨原发性高血压（essential hypertension,EH）不同中医证型与血管紧张素原（angiotensino-gen,AGT）M235T基因多态性的相关性。方法选择EH患者120例,辨证分型分别为肝火亢盛型、阴虚阳亢型、阴阳两虚型、痰湿壅盛型,并选取正常人30例作为对照组,采用聚合酶链反应（polymerase chain reac-tion,PCR）检测AGTM235T基因多态性。结果 EH组MM、MT基因型频率和M等位基因频率显著低于健康对照组（P〈0.05）,而TT基因型频率和T等位基因频率显著高于健康对照组（P〈0.05）;EH肝火亢盛型AGTM235T基因TT型频率及等位基因T频率显著高于健康对照组（P〈0.05）;EH组各证型基因型频率和等位基因频率比较,差异均无统计学意义（P〉0.05）。结论 EH肝火亢盛型可能与AGTM235T基因TT型有关联。

Relationship between Polymorphism of Angiotensinogen M235T Gene and Traditional Chinese Medicine Syndromes in Essential Hypertension

Objective To study the polymorphism of angiotensinogen（AGT） gene M235T and its relationwith traditional Chinese medicine（TCM） syndromes in essential hypertension（EH）.Methods One hundred and twenty EH patients were diagnosed as four syndrome types based on syndrome differentiation principle in TCM,i.e.,liver-fire hyperactivity syndrome,yin-deficiency and yang-hyperactivity syndrome,sputum-dampness retention syndrome,and yin-yang deficiency syndrome,and another 30 healthy volunteers were selected as controls.The polymerase chain reaction（PCR） was used to test AGT M235T polymorphism.Results The frequencies of MM and MT genotypes,and M allele in EH patients were significantly lower than those in healthy controls（P〈0.05）;while the frequencies of TT genotype and T allele were significantly higher than those in healthy controls（P〈0.05）.The frequencies of TT genotpye and T allele in EH patients with liver-fire hyperactivity syndrome were significantly higher than those in healthy controls（P〈0.05）.There was no significant difference in the frequencies of genotypes and alleles among four syndrome types in EH patients（P〉0.05）.Conclusion The TT genotype of AGT M235T gene might be associated with liver-fire hyperactivity syndrome in EH patients.