人乳腺癌MCF-7/ADM细胞的耐药谱及brca1基因表达的实验研究

目的]研究人乳腺癌MCF-7/ADM细胞的耐药谱并探讨brca1基因产物表达与该细胞耐药性的相关性。方法]经MTT法检测MCF-7/ADM细胞的耐药谱;通过免疫荧光法检测brca1基因产物表达情况。结果]测得MCF-7/ADM细胞对阿霉素的耐药倍数为3.7倍;对长春新碱的耐药倍数为70.3倍;对氟尿嘧啶的耐药倍数为7.8倍。brca1基因产物在MCF-7/ADM细胞中的表达强度(98.23±7.63)高于MCF-7/S细胞(8.69±1.25),差异具有显著性意义(P<0.01)。结论]MCF-7/ADM细胞是多药耐药细胞株,brca1与肿瘤多药耐药产生可能有一定的相关性。

Study drug-resistant spectrum of MCF-7/ADM and expression of brca1

Objective] To study the drug-resistant spectrum of MCF-7/ADM cell and the relationship of the cells to brca1 protein expression.Methods] The drug-resistant spectrum of MCF-7/ADM cell was studied by MTT assay,and then the expression of brca1 protein was detected by immunofluoresence assay.Results] The drug-resistant folds to ADM,VCR,and 5-FU respectively were 3.7,70.3,and 7.8.The expression of brca1 gene was high in MCF-7/ADM cells(98.23±7.63)and low in MCF-7/S cells(98.23±7.63),P<0.01.Conclusion] MCF-7/ADM cell is multidrug resistance cell.There may be a relationship between brca1 and MDR.