Aurora-A激酶在肺癌中的表达

目的]探讨Aurora-A基因在不同组织类型肺癌中的表达及其与临床的相关性.方法]采用RT-PCR、Western-blot与免疫组化方法检测肺癌组织标本行Aurora-A的mRNA与蛋白表达.结果]肺癌30例标本中RT-PCR检测Aurora-A mRNA的表达,19例(63%)肺癌表达水平高于相应癌旁组织,其中Western blot方法检测20例中,13例(65%)蛋白表达水平高于相应癌旁组织.免疫组化检测48例肺癌中32例高表达(66.67%).肺癌Aurora-A mRNA的表达水平与肺癌患者的性别,吸烟史以及病理特征无明显相关性.肺鳞癌的Aurora-A的蛋白水平高表达程度与肿瘤转移有相关性.结论]肺癌组织Aurora-A表达明显高于其癌旁组织,提示Aurora-A基因的过表达与肺癌的发生有相关性.

Expression of aurora-A kinase in different human lung cancer

Objective] To study Aurora-A gene expression status of lung cancer and its influence on genesis of lung cancer in vivo.Methods] The levels of Aurora-A mRNA and protein of lung cancer samples were detected by RT PCR,Western Blot or/and immunohistochemistry respectively.Results] In 30 cases of lung cancer patients,the over-expressions of Aurora-A mRNA were observed in 19 cases(63%) compared with neighboring tissues(P<0.05).Western blot was adopted to assess the status of Aurora-A protein expressed in tumor in vivo from 20 patients.The over-expressioof Aurora-A protein n was detected in 13 cases(65%) compared with their neighboring tissue(P<0.05).Correspondingly,over expression were also found in 32 cases(66.67%)of 48 cases dectected by immunohistochemistry.No significant correlation existing between over expression of Aurora-A mRNA and sex,smoking history and pathological characteristics was found.On the contrary,correlation was observed between over expression level of Aurora-A protein and metastases in 48 squamous lung cancer patients.Conclusion] The Aurora-A is highly expressed in lung cancer tissue than in neighboring tissue,suggesting the over expressed Aurora-A be one of oncogenetic factors of lung cancer.