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SDS—PAGE分离、制备蛋白质的一种简易方法
引用本文:李宾,燕秋,马克里,朱正美.SDS—PAGE分离、制备蛋白质的一种简易方法[J].大连医科大学学报,1992(4).
作者姓名:李宾  燕秋  马克里  朱正美
作者单位:大连医学院生化教研室,大连医学院生化教研室,大连医学院生化教研室,大连医学院生化教研室
摘    要:SDS-PAGE做为一种蛋白质及核酸分析的手段已得到广泛应用。蛋白质经凝胶电泳分离后,再通过电泳将凝胶上特异条带洗脱下来也是蛋白质分离、纯化的一种有效方法,但该法的推广使用受到仪


ISOLATION AND PREPARATION OF PROTEIN BY A SIMPLE METHOD OF SDS-PAGE
Li Bin,et al..ISOLATION AND PREPARATION OF PROTEIN BY A SIMPLE METHOD OF SDS-PAGE[J].Journal of Dalian Medical University,1992(4).
Authors:Li Bin  
Institution:Li Bin,et al.Department of Biochemistry
Abstract:A simple method of isolation and preparation protein from rabbit endometrium has been presented. Crude extractive proteins were seperated by SDS-PAGE. The gel slice containln the specific protein band was cut and eluted by the specially deeigned Eletro-eluting device. In addition, we found that when the bands fade with commonly used Coomassie blue R-250 staining, the same gel can be stained with silver staining and the satisfactory result will be achieved.
Keywords:protein isolation preparation  sodium ddecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)  Stain
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