房颤射频消融术前后血浆miR-328表达变化

目的 探讨血浆miR-328在不同类型房颤患者的射频消融术前后的表达变化,为进一步探讨房颤发病机制提供新方向.方法 选取从2015年3月至2016年1月期间在大连医科大学附属第一医院行射频消融术的心房颤动患者60例作为房颤组,选择同期大连医科大学附属第一医院体检中心的健康体检者20例作为正常对照组.使用实时荧光定量PCR技术检测正常对照组及不同类型房颤组手术前后血浆miR-328表达水平的变化.结果 房颤组血浆miR-328同对照组比较表达上升.血浆miR-328在房颤组射频消融术前后相对表达量分别为2.04±0.31和1.12±0.06,术后同术前比较表达下降,差异均有显著性意义,P＜0.05;血浆miR-328在阵发性房颤、持续性房颤和永久性房颤中相对表达量分别为1.85±0.23、2.13±0.36和2.72±0.93,表达逐渐升高,组间比较差异均有统计学意义,P ＜0.05.结论 miR-328同房颤病情发展密切相关,可能参与调控房颤的发病机制.

Plasma miR-328 expression changes and clinical significance in patients with atrial fibrillation before and after radiofrequency ablation

Objective To investigate the changes of plasma miR-328 expression levels before and after radiofrequency ablation(RFA) and its correlation with different clinical atrial fibrillation(AF) types and explore the pathogenesis of atrial fibrillation.Methods The plasma miR-328 expression levels were detected in 60 AF patients before and after RFA and 20 healthy PE subjects using real-time fluorescence quantification PCR technique.Results The plasma miR-328 level in atrial fibrillation group was higher than that in control group.The relative expression levels of plasma miR-328 in the AF group before RFA (2.04 ±0.31) was higher than that after RFA (1.12 ±0.06),P ＜0.05.The relative expression levels of plasma miR-328 were 1.85 ± 0.23,2.13-± 0.36 and 2.72 ±-0.93 in paroxysmal AF,persistent AF and permanent AF,respectively (P ＜ 0.05).Conclusion The plasma miR-328 expression level are associated with AF progression,which would be a prognosis marker of AF treatment.