慢性乙型肝炎患者血清e系统与HBV-DNA含量的关系及临床意义

目的]探讨慢性乙型肝炎患者血清HBV-DNA的表达水平与e系统的关系及其与肝细胞损害的关系.方法]用实时荧光定量PCR方法检测乙肝患者血清HBV-DNA,同时用ELISA法检测血清乙肝标志物.结果]HBeAg（＋）组与抗-HBe（＋）组HBV-DNA阳性率分别为100%、58.89%,两组HBV-DNA含量比较,差异无显著性（P＞0.05）;两组肝细胞损害指标ALT、AST、TBA比较,差异有显著性（P＜0.05）,HBV-DNA含量与肝细胞损害各指标均无相关性.结论]HBV-DNA的定量检测可更准确地反映体内HBV增殖复制水平,HBV-DNA的含量与肝实质损害程度无关.

Relation and clinical meaning of serum e system and HBV-DNA levels in patients with chronic hepatitis B

Objective]To explore the relationship between the expression levels of serum HBV-DNA and the serum e system,and the degree of hepatocyte injury in patients with chronic hepatitis B.Methods]The contents of HBV-DNA were detected by fluorescence quantitative PCR,and the HBV serum marks of immunology by ELISA.Results]The positive rate of HBeAg(+) group and anti-HBe(+) group was respectively 100% and 58.89%,The contents of HBV-DNA copies had no significant difference between two groups(P>0.05);There had significant difference between the contents of HBV-DNA copies and the severe degree of hepatocyte injury(P<0.05);The contents of HBV-DNA copies had no correlation with the severe degree of hepatocyte injury(P>0.05).Conclusion]The quantitative detection of serum HBV-DNA can accurately react the duplication level of hepatitis virus.The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV-DNA copies.