DOT—ELISA对斯氏肺吸虫病的免疫诊断研究

用DOT－ELISA（斑点－酶联免疫吸附试验）对20例斯氏肺吸虫病进行检测，19例阳性反应，阳性检出率为950％，26例对照血清中，12例日本血吸虫病，2例阳性，交叉反应率16．9％．同时，对DOT－ELISA的特异性，敏感性进行研究，32例其他血吸虫病和肺部疾病血清经该法检验，出现交叉反应率为12．5％．24例可疑斯氏肺吸虫血清分别用DOT－ELISA和平板－ELISA检测，阳性总符合率为85．7％．本文认为DOT－ELISA具有较高的特异性、敏感性，成本低,操作简便，观测结果直接，为期氏肺吸虫病免疫诊断的较好方法．

STUDY ON IMMUNODIAGNOSIS OF PARAGONIMIASIS BY DOT-ELISA

This paper reportes that a dot-enzyme-linked immunosorbent assay (dot-ELISA)was developed for immunediagnosis of 20 cases of Paragonimiasis. The positive rate was 95% (19/20 ). In 26 control sera, including 12 cases of schistosomiasis, 2 cases of schistosomiasis were positive,the cross reaction rate was 16. 7 %. In order to test the sensitivity and specificity of Dot-ELISA, seraof 32 patients, of other flukes infections and pulmonary disecases were detected by this method. Thecross reaction rate was 12. 5%. When compared with ELISA in 24 suspected patients, the positiverates of the two methods were not significantly different (coincident rate of 85. 7% ). Dot-ELISA appears to be sensitive. specific, inexpensive, easy to perform, and stable for immunediagnosis ofParagonimiasis.