血管紧张素-（1-7）对TGF-β1诱导的大鼠心肌成纤维细胞Smad3、Smad7 mRNA表达的影响

目的：观察血管紧张素-（1—7）对TGF-β1诱导幼年大鼠心肌成纤维细胞增殖以及Smad3、Smad7mRNA表达的影响。方法：分离培养SD仔鼠心脏成纤维细胞，以Ang-（1—7）、TGF-β1、Ang-（1—7）＋TGF-β1组、TGF-βI型受体抑制剂等干预，通过WST-1法检测细胞增殖，RT—PCR测定心脏成纤维细胞Smad3、Smad7mRNA的表达。结果：①与空白对照组比较，TGF-β1组心脏成纤维细胞的OD值增加（P〈0．05）；与TGF-β1组比较，0．001～1．0μmol／L血管紧张素－（1-7）和5μg／TGF-β1共同干预后OD值逐渐降低（P〈0．05）。②与对照组比较，TGF-β1组心脏成纤维细胞的Smad3mRNA表达升高（P〈0．05），Smad7mRNA表达降低（P〈0．05）。结论：血管紧张素-（1—7）能有效抑制TGF-β1引起的心脏成纤维细胞的增殖，其作用不通过TGF-βI型受体介导。

EFFECT OF ANGIOTENSIN-(1-7)ON SMAD3 AND SMAD7 MRNA EXPRESSION OF RAT CARDIAC FIBROBLASTS INDUCED BY TGF-β1

Objective： To investigate the effect of angiotensin -（1-7） Ang-（1-7）] on cell proliferation and the expression of Smad3 and Smad7 mRNA of cardiac fibroblasts （CF） induced by TGF-β1. Methods： CF were isolated and cultured from Neonatal SD rats.The cells were divided into different groups and treated with Ang-（1- 7）, TGF-v1, TGF-β1＋ Ang-（1-7）, and the acceptor inhibiter of TGF-β1 respectively. Cell number was evaluated by WST-1. Smad3 and Smad7 mRNA expression in CF were analyzed by RT-PCR. Results： ①Compared with the control group, 5μg/L TGF-β1 increased absorption value of CF（P〈0.05）. Compared with 5μg/L TGF-β1 group, TGF-β1＋ 0.001 -1.0μmol/L Ang-（1-7） attenuated the absorption value of CF in a concentration dependent manner （P〈0.05）. ②Compared with TGF-β1 group, the expression of Smad3 mRNA decreased, while the expression of Smad7 mRNA increased in TGF-β1＋Ang-（1-7） group （P〈0.05）. Conclusion： Ang-（1-7） can downregulate the expression of Smad3 mRNA, and up-regulate the expression of Smad7 mRNA induced by TGF-β1.