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热休克蛋白72的克隆表达及其对人脐静脉内皮细胞ECV304凋亡的影响
引用本文:刘兴晖,祝成亮,宋惠,李毅,刘芳.热休克蛋白72的克隆表达及其对人脐静脉内皮细胞ECV304凋亡的影响[J].郧阳医学院学报,2009,28(3):222-225.
作者姓名:刘兴晖  祝成亮  宋惠  李毅  刘芳
作者单位:刘兴晖,宋惠,李毅,LIU Xing-hui,SONG Hui,LI Yi(郧阳医学院附属东风医院检验科,湖北十堰,442008);祝成亮,刘芳,ZHU Cheng-liang,LIU Fang(武汉大学生命科学学院病毒学国家重点实验室,湖北武汉,430072) 
基金项目:湖北省教育厅重点科研项目 
摘    要:目的:克隆并表达热休克蛋白72(HSP72),探讨其在动脉粥样硬化患者血清中的表达及其对细胞凋亡的影响。方法:构建HSP72原核表达载体,纯化并制备相应的抗体;Westernblotting检测其在动脉粥样硬化患者血清中的表达;四甲基偶氮唑盐(MTT)法和流式细胞术分析HSP72对人脐静脉内皮细胞ECV304细胞增殖和凋亡的影响。结果:表达并纯化了HSP72蛋白,制备了其相应的多克隆抗体;HSP72在动脉粥样硬化患者血清中表达明显升高;对ECV304细胞增殖起到一定的抑制作用,同时能促进细胞凋亡。结论:HSP72可能通过促进细胞凋亡,从而促进动脉粥样硬化发生。

关 键 词:动脉粥样硬化  热休克蛋白72  克隆  凋亡

Cloning and Expression of Heat Shock Protein 72 and Its Effect Oil Apoptosis of Human Umbilical Vein Endothelial Cells ECV304
LIU Xing-hui,ZHU Cheng-liang,SONG Hui,LI Yi,LIU Fang.Cloning and Expression of Heat Shock Protein 72 and Its Effect Oil Apoptosis of Human Umbilical Vein Endothelial Cells ECV304[J].Journal of Yunyang Medical College,2009,28(3):222-225.
Authors:LIU Xing-hui  ZHU Cheng-liang  SONG Hui  LI Yi  LIU Fang
Abstract:Objective To clone and express heat shock protein 72(HSP72),and to observe its expression in serum of patients with atherosclerosis and investigate its effect on cell apoptosis.Methods The prokaryotic expression vector of HSP72 was constructed,purifed and its polyclonal antibodies were prepared;HSP72 expression in serum of patients with atherosclerosis was determined with Western blotting;the effects of HSP72 on the proliferation and apoptosis of ECV304 were analyzed with MTT and flow cytometry.Results HSP72 was expressed,purifed and its polyclonal antibodies were prepared;HSP72 expression was significantly increased in serum of patients with atherosclerosis;HSP72 inhibited ECV304 proliferation and promoted accelerated cell apoptosis.Conclusion HSP72 may promote the pathogenesis of atherosclerosis by promoting endothelial cell apoptosis.
Keywords:Atheroselerosis  Heat shock protein 72  Clone  Apoptosis
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