不同临床病理特征涎腺黏液表皮样癌中CEACAM 5与PCNA的表达及对预后的影响

［摘要］ 目的 研究癌胚抗原相关细胞黏附分子5（carcinoembryonic antigen related cell adhesion molecule 5，CEACAM 5）及增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）在涎腺黏液表皮样癌(mucoepidermoid carcinoma，MEC)中的表达情况，并研究其表达与肿瘤患者临床预后的关系。 方法 应用免疫组织化学链霉菌抗生物素-过氧化物酶连接（streptavidin-perosidase，SP）法检测55例不同临床病理特征的涎腺MEC组织中CEACAM 5、PCNA的表达情况，30例涎腺多形性腺瘤（salivary pleomorphic adenoma，SPA）及30例癌旁正常涎腺组织作为对照；分析CEACAM 5、PCNA表达的相关性及其与临床预后的关系。 结果 MEC组CEACAM 5、PCNA表达阳性率明显高于SPA组和对照组（P＜0.01）。病理分级Ⅲ级涎腺MEC组织中CEACAM 5、PCNA表达阳性率均明显高于病理分级Ⅰ+Ⅱ级，TNM 分期Ⅲ+Ⅳ期涎腺MEC组织中CEACAM 5、PCNA表达阳性率均明显高于TNM 分期Ⅰ+Ⅱ期，有淋巴结转移涎腺MEC组织中CEACAM 5、PCNA表达阳性率均明显高于无淋巴结转移者( P＜0.01)；不同性别、年龄、部位涎腺MEC组织中CEACAM 5、PCNA表达阳性率差异均无统计学意义(P＞0.05)。涎腺MEC组织中CEACAM 5和PCNA的表达呈正相关（P＜0.05）。涎腺MEC组织中CEACAM 5与PCNA表达阴性者的生存时间均明显长于表达阳性者（P＜0.05）。 结论 CEACAM 5和PCNA在涎腺MEC组织中异常高表达，能够协同调控肿瘤的增殖，影响肿瘤的生物学活性，最终影响肿瘤患者的预后。

Expression and influence of CEACAM 5 and PCNA in mucinous epidermoid carcinoma of the parotid gland with different clinical pathological features on clinical prognosis

［Abstract］Objective〖HTSS〗To study the expression of carcinoembryonic antigen related cell adhesion molecule 5（EACAM 5） and proliferating cell nuclear antigen（PCAN） in mucoepidermoid carcinoma（MEC） of salivary glands, and to investigate correlation with clinicopathological and prognosis of patients. 〖WTHZ〗Methods〖HTSS〗The immunohistochemical streptavidin-perosidase（SP）was used to examine the expression of CEACAM 5 and PCNA in 55 MEC of salivary glands with different clinicopathological characteristics, 30 salivary pleomorphic adenoma（SPA）and 30 tumor adjacent normal tissues（control group）. The relationship of CEACAM 5, PCNA and clincal prognosis was analyzed. 〖WTHZ〗Results〖HTSS〗Compared with SPA and control group, the positive expression of CEACAM 5 and PCNA were higher in MEC tissues（P＜0.01）. In MEC of salivary glands group, the positive level of CEACAM 5 and PCNA in pathological grade Ⅲ. The positive rates of CEACAM 5 and PCNA in MEC of salivary gland in TNM stage Ⅲ+Ⅳ were significantly higher than those in TNM stage Ⅰ+Ⅱ. The positive rates of CEACAM 5 and PCNA in MEC with lymph node metastasis were significantly higher than those without lymph node metastasis(P＜0.01). There were no significant difference among the positive expression of CEACAM 5 and PCNA in gender, age and tumor sites（P＞0.05）. The expression of CEACAM 5 was positive related to the level of PCNA in MEC tissues（P＜0.05）. The overall survival of the negative expression of CEACAM 5 and PCNA was significantly longer than the positive expression of CEACAM 5 and PCNA（P＜0.05）. 〖WTHZ〗Conclusion〖HTSS〗CEACAM 5 and PCNA are highly expressed in MEC of salivary gland, which can co regulate the proliferation of tumor, affect the biological activity of tumor, and ultimately affect the prognosis of tumor patients.