贝那普利对糖尿病大鼠肾脏的保护作用

目的探讨贝那普利(benazepril)对糖尿病大鼠肾脏病变的保护机制。方法采用健康雄性Wister大鼠42只,随机分为正常组、糖尿病组和贝那普利治疗组。实验结束后,取大鼠的尿、血、肾组织标本,行生物化学和聚合酶链反应(polymerasechainreaction,PCR)检测。结果大鼠体质量、肾质量/体质量、血压、血糖,24h尿蛋白定量、血肌酐,尿素氮各组间均有统计学意义(P<0.05);肾组织降钙素基因相关肽(calcitoningene relatedpeptide,CGRP)和内皮素(endothelin,ET)mRNA表达均有统计学意义(P<0.01)。结论贝那普利通过抑制血管紧张素转化酶(angiotensin convertingenzyme,ACE)而减少ET1分泌,促进CGRP合成,从而减轻CGRP与ET1的失衡,对大鼠糖尿病肾脏病变有明确的保护作用。

PROTECTING EFFECT OF BENAZEPRIL IN DIABETIC NEPHROPATHY OF RATS

ObjectiveTo investigate the protecting mechanisms of benazepril in diabetic nephropathy rats. MethodsForty-two health male Wister rats were randomly divided into normal group, diabetic nephropathy group and benazepril group. After experiment urine, blood, kidney, samples were taken, and biochemistry index and PCR were performed. ResultsRat body mass, kidney mass/body mass, blood pression, blood sugar, urine protein in 24 h, serum creatinine(Scr), blood urea nitrogen(BUN) had distinct difference between three gruops(P< 0.05). Expression of calcitonin-gene-related peptide(CGRP) mRNA and endothelin(ET)in kidneys had distinct difference between three gruops(P< 0.01). ConclusionBy restraining angiotensin converting enzyme, benazepril restrains secreting of ET, accelerates CGRP compound, lightens the unbalance of CGRP and ET, so benazepril has clearly protecting effect in diabetic nephropathy.