运动状态下骨骼肌细胞线粒体膜电位的变化与细胞凋亡

目的 研究运动大鼠骨骼肌细胞线粒体膜电位的变化与细胞凋亡的关系。方法 用流式细胞仪检测肌细胞线粒体膜电位、ＤＮＡ倍体分析及脱氧核糖核苷酸末端转移酶介导的缺口末端标记技术。结果 线粒体膜电位在游泳运动后第１周降低，第２周后开始明显增高，第３周略有降低，运动后各组的ＤＮＡ均出现亚“Ｇ１”峰即凋亡峰。ＴＵＮＥＬ显示，运动后第１周凋亡比例最高，第２周后比例降低。结论 运动引起线粒体膜电位的改变，该方法快速

MITOCHONDRIAL MEMBRANE POTENTIAL CHANGE AND APOPTOSIS OF MUSCULAR CELL DURING EXERCISE

ObjectiveTo investigate the relationship between mitochondrial membrane potential change, and apoptosis of muscular cells in rats after exercise.MethodsMitochondrial membrane potential in the muscular cell was determined with analysis of DNA double by the technique of flow cytometry,and terminal deoxynucleotidyl transferase d UTP nick end labelling(TUNEL) test was conducted. ResultsThe mitochondrial membrane potential decreased in one week, then increased markedly in two weeks, and it become much lower compared with control by three weeks after exercise. DNA double analysis demonstrated that sub G 1 namely apoptosis peak appeared at different level in three groups after exercise. TUNEL showed that the apoptosis rats augmented after exercise compared with control rats, and the rate reached the highest value after one week.ConclusionExercise may cause mitochondrial membrane potential change and induce muscular cell apoptosis.The procedures of these measurements are performed quickly with high specificity and sensitivity.