大肠癌中错配修复酶hMLH1、hMSH2及环氧化酶-2表达的研究

目的观察大肠癌中环氧化酶 2 (cyclooxygenase 2 ,COX 2 )、人类mut s同系物 1 (humanmut lhomologue 1 ,hMLH1 )、人类mut s同系物 2 (humanmut shomologue 2 ,hMSH2 )的表达。 方法应用COX 2、hMLH1、hMSH2多克隆抗体 ,采用免疫组织化学方法检测 78例大肠癌手术切除标本中COX 2、hMLH1、hMSH2蛋白的表达情况。结果在所检测的 78例大肠癌组织中 ,hMLH1表达的缺乏率为 2 9.4 9% ,hMSH2表达的缺乏率为 2 8.2 1 % ,COX 2表达阳性率为 74 .36 %。结论在大肠癌中hMLH1、hMSH2蛋白均有一定的缺乏率 ,一部分大肠癌中COX 2呈阳性表达 ,提示大肠癌的发生至少有 2种可能的途径即错配修复酶缺陷和COX 2过表达

EXPRESSION OF MISMATCH REPAIR ENZYME hMLH1,hMSH2 AND CYCLOOXYGENASE-2 IN COLORECTAL CANCER

ObjectiveTo investigate the expression of cyclooxygenase 2 (COX 2),mismatch repair enzyme human mut l homologue 1(hMLH1),human mut s homologue 2(hMSH2) in colorectal cancer. MethodsThe expression of COX 2, hMLH1 and hMSH2 protein was analyzed in 78 colorectal cancer by using polyclonal antibody of COX 2,hMLH1 and hMSH2 with immunohistochemistry method. ResultsIn the 78 colorectal cancer samples, the negative rates of hMLH1 and hMSH2 were 29.49 % and 28.21 % , respectively. The positive rate of COX 2 was 74.36 % . ConclusionIn colorectal cancer, there was negative expression of hMLH1 and hMSH2,and a part of colorectal cancer demonstrated positive expression of COX 2,which implied that there were possibly at least two pathways to colorectal cancer: mismatch repair enzyme deficiency and high expression of COX 2.