| 中药水蛭对缺血/再灌注后脑细胞的抗凋亡作用 |
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| 引用本文： | 曹翠丽,马常升,马文领,杨天祝.中药水蛭对缺血/再灌注后脑细胞的抗凋亡作用[J].河北医科大学学报,2000,21(4):193-195. |
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| 作者姓名： | 曹翠丽 马常升 马文领 杨天祝 |
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| 作者单位： | 1. 河北医科大学基础医学研究所神经生物学研究室,石家庄,050017
2. 中国人民解放军白求恩军医学院解剖学教研室 |
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| 摘 要： | 目的 观察水蛭水煎醇提取液对大鼠所血／再灌注后脑细胞凋亡的保护作用。方法 将雄性ＳＤ大鼠６０只，随机分为为单纯缺血／再灌注组（Ａ组）和缺血／再灌注＋水蛭治疗组（Ｄ组）。插线法制备大鼠大脑中动脉阻塞／再灌注模型，用流式细胞仪检测两组的脑细胞凋亡率。结果 Ｂ组脑细胞凋亡率明显低于Ａ组（Ｐ〈０．０５），且脑细胞凋亡的率明显你于Ａ组（Ｐ〈０．０５），且脑细胞凋亡的发生推迟。结论水蛭水煎醇提取液可降低缺血／
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| 关 键 词： | 水蛭 药理学 再灌注损伤 脑细胞 抗凋亡作用 |
ANTI-APOPTOTIC EFFECT OF TRADITIONAL CHINESE MEDICINE HIRUDO ON CEREBRAL CELLS AFTER OCCLUSION/REPERFUSION |
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| Cao Cuili,Ma Changsheng,Ma Wenling,Yang Tianzhu.ANTI-APOPTOTIC EFFECT OF TRADITIONAL CHINESE MEDICINE HIRUDO ON CEREBRAL CELLS AFTER OCCLUSION/REPERFUSION[J].Journal of Hebei Medical University,2000,21(4):193-195. |
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| Authors: | Cao Cuili Ma Changsheng Ma Wenling Yang Tianzhu |
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| Institution: | Shijiazhuang 050017 |
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| Abstract: | ObjectiveTo investigate the anti apoptotic effect of hirudo on cerebral cells after middle cerebral artery (MCA) ischemia/reperfusion in rats. MethodsMale SD rats (n=60) randomly divided into ischemia/reperfusion group (group A) and ischemia/reperfusion treatment with hirudo group (group B).The MCA of SD rats was occluded for 2 h by inserting an intraluminal monofilament, and reperfusion was instituted for 6 h, 12 h, 24 h, 48 h, 72 h, 96 h (n=5 group B) respectively.Apoptotic rates of both groups were measured by flow cytometry.ResultsApoptotic rate of cerebral cells of group B significantly lowered than group A (P< 0.05 ), and the peak of apoptotic rate was postponed. ConclusionHirudo can significantly reduce the apoptotic rate of cerebral cells after ischemia/reperfusion, thereby protect cerebral cells from apoptosis. |
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| Keywords: | hirudo/pharmacol reperfusion injury/metab brain apoptosis/physiol flow cytometry/methods |
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