| 大鼠口服知母皂苷后入血成分研究 |
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| 引用本文： | 梁均乐,韩丽萍,蒋琳兰.大鼠口服知母皂苷后入血成分研究[J].广州医药,2010,41(3):69-72. |
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| 作者姓名： | 梁均乐 韩丽萍 蒋琳兰 |
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| 作者单位： | 1. 华南理工大学生物科学与工程学院,广州,510006
2. 广州军区广州总医院药剂科,510010 |
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| 摘 要： | 目的 分析大鼠口服知母总皂后吸收入血的情况，为进一步研究知母总皂苷的药理作用提供化学物质依据。方法给大鼠灌胃知母皂苷2．5g／kg，于不同时间采集血清样品，用电喷雾质谱（ESI—MS）检测知母皂苷吸收入血成分。结果通过比较给药前后的TOF总离子流图，对给药后血浆中特征峰的保留时间及相关化合物离子峰进行了比较分析，在血浆中发现了两个原型皂苷。结论大鼠口服知母总皂苷后，知母皂苷B-Ⅱ和知母皂苷B-Ⅲ被吸收入血。
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| 关 键 词： | 知母皂苷 入血成分 电喷雾质谱 |
Identification of constituents in rat serum by HPLC-MS following oral administration of saponins from Anemarrhena asphodeloidess |
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| Liang Junle,Han Liping,Jiang Linlan.Identification of constituents in rat serum by HPLC-MS following oral administration of saponins from Anemarrhena asphodeloidess[J].Guangzhou Medical Journal,2010,41(3):69-72. |
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| Authors: | Liang Junle Han Liping Jiang Linlan |
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| Institution: | ( School of Bioscience and Bioengineering, South China University of Technology, Gnangzhou 510006, China) |
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| Abstract: | Objective To investigate the constituents of saponins from Anemarrhena asphodeloides absorbed into rat serum after oral administration. Methods Serum was collected after oral administration of 2. 6 g/kg saponins from Anemarrhena asphodeloides to rats. The sample was detected by ESI-MS. Results A total of two characteristic HPLC peaks in the TIC chromatograms were found based on comparing total ion chromatograms of the blank with the metalolic serum as well as mass spectra. Conclusion Timosaponin B- Ⅱ and timosaponin B-Ⅲ were absorbed into serum after oral administration of saponins from Anemarrhena asphdeloides. |
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| Keywords: | Saponins of Anemarrhena asphodeloides Constituents in rat serum Electronspurtion mass spectroscopy （ESI-MS） |
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