1型和2型糖尿病大鼠肺组织脂联素受体的表达

目的观察1型糖尿病（1-DM）和2型糖尿病（2-DM）大鼠肺组织脂联素受体（AdipoRl和AdipoR2）的表达情况。方法sD大鼠随机分为1一DM组（一次性腹腔内注射高剂量链脲佐菌素建模，普通饮食，8周后处死）、72h组（一次性腹腔内注射高剂量链脲佐菌素建模，普通饮食，72h后处死）、2-DM组（高脂饮食配合一次性腹腔内注射低剂量链脲佐菌素建模，8周后处死）和对照组（一次性腹腔内注射等容量枸橼酸缓冲液，普通饮食）。分别检测血清胰岛素和脂联素水平以及肺组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性以及AdipoRl和AdipoR2的表达，HE染色观察肺组织病理学改变。结果与对照组比较，1-DM组和2-DM组血清脂联素水平均显著降低（P〈0．05）；2-DM组血清脂联素水平显著低于1-DM组（P〈0．05）；1-DM组和2-DM组肺组织AdipoRl表达及SOD活性均显著低于对照组，而72h组与对照组比较差异无统计学意义（P〉0．05）；1一DM组和2-DM组肺组织有基膜增厚、血管堵塞等病理学改变。结论肺组织脂联素受体表达以AdipoRl为主，高血糖可引起肺损伤、肺AdipoRl表达及SOD活性降低。

Expressions of adiponectin receptors in lung tissue of type 1 and type 2 diabetic rats

Objective To investigate expressions of adiponectin receptors （AdipoR1, AdipoR2） in lung tissue in type 1 and type 2 diabetes mellitus rats induced by streptozotocin （STZ）. Methods A total of 40 SD rats were randomly divided into 4 groups： control （Con） group, type 1 diabetes mellitus （1-DM） group, 72 h after STZ injection （72 h） group, and type 2 diabetes mellitus （2-DM） group. The rats in 1-DM group and 72 h group were injected STZ intraperitoneally with a dose of 60 mg/kg while the ones in Con group were only done with the same dose of citric acid buffer. The rats in 2-DM group were injected STZ intraperitoneally with a dose of 35 mg/kg. Serum insulin and adiponectin levels were evaluated by ELISA. The content of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in lung tissue were measured. Expression of AdipoR1, AdipoR2 in lung tissue was determined by immunohistochemistry. The pathological changes of lung tissue were examined by HE staining under light microscope. Results The levels of serum adiponectin in 1-DM and 2-DM groups were lower than that in Con group （P 〈0.05）. The level of serum adiponectin in 2-DM group was lower than that of 1-DM group （P 〈 0.05）. Compared with Con group, the expression of AdipoR1 and activity of SOD in lung tissue in 1-DM and 2-DM groups were lower while those in 72 h group were similar. In 1-DM and 2-DM groups, the rat lung tissue manifested injuries such as basement membrane thickening and blood vessel occlusion. Conclusion Adiponectin receptors expression in lung tissue is given priority to AdipoR1. High blood glucose may result in lung injury and decrease AdipoR1 expression and SOD activity.