肠壁穿刺逆行胰胆管注射牛黄胆酸钠重症急性胰腺炎造模

目的建立一种操作简单、病变典型且稳定性好的大鼠重症急性胰腺炎(SAP)模型。方法46只健康雄性SD大鼠分为SAP组和对照组,SAP组经肠壁穿刺逆行胰胆管注射5%牛黄胆酸钠,对照组同法注射等量0.9%生理盐水。两组大鼠分别于注射后3、6、12 h检测血清淀粉酶水平、胰腺组织的湿/干质量比率及病理组织学评分,观察24 h生存指数。结果SAP组各时间段血清淀粉酶和胰腺病理组织学评分均显著高于对照组,胰腺组织湿/干质量比率在注射后6、12 h显著高于对照组;其造模后24 h的总死亡率为70%(7/10)。结论经肠壁穿刺逆行胰胆管注射牛黄胆酸钠成功诱发大鼠SAP,其模型操作简单、可重复性好。

Establishing Severe Acute Pancreatitis Model on Rats by Retrograde Injection of Sodium Taurocholate into Biliopancreatic Duct through Duodenal Wall

Objective To establish a simple,typical,reliable model for severe acute pancreatitis(SAP) on rats.(Methods)The SAP model was developed in rats by retrograde injection of 5% sodium taurocholate into biliopancreatic duct through duodenal wall.The rats were sacrificed at 3,6,and 12 h,respectively,after the onset of induction.Serum amylase and the wet/dry weight ratio of pancreas were measured.The pancreatic histology and the 24-hour survival rate were also evaluated. Results The levels of serum amylase,histological score and the wet/dry weight ratio of pancreas in SAP group were significantly higher than those of the control group at each time point(P<0.01,respectively).The 24 h mortality was about 70%. Conclusion Retrograde injection of 5% sodium taurocholate into biliopancreatic duct through duodenal wall can establish a simple and reliable SAP model on rats.