寻常型天疱疮皮损组织中Th17细胞相关细胞因子的表达

目的 研究Th17细胞相关细胞因子IL-23和IL-17在寻常型天疱疮（PV）发病机制中的作用.方法 采用免疫荧光染色法分析PV患者皮损组织和健康人皮肤组织中Th17细胞相关因子IL-17和IL-23的表达及CD163~＋巨噬细胞的分布.结果 IL-23~＋细胞的染色与CD163~＋巨噬细胞的染色重叠.PV患者真皮中IL-23~＋ CD163~＋细胞和IL-17~＋细胞在每个视野（×200）中的平均数分别为80.17±5.42和48.17±8.03,而正常人真皮中分别为29.50±4.45和9.50±3.35,两组差异均有统计学意义（P〈0.01）.真皮层中IL-23~＋细胞数和IL-17~＋细胞数存在相关性（r=0.945,P〈0.001）.结论 CD163~＋巨噬细胞是IL-23重要分泌源;IL-23和IL-17在PV患者皮损组织中高表达,IL-23/lL-17细胞因子轴在PV发病机制中可能起着非常重要的作用.

Expression of Th17 cell associated cytokines in pemphigus vulgaris lesions

Objective To investigate the role of Thl7 cell associated cytokines of IL-23 and IL-17 in the pathogenesis of pemphigus vulgaris. Methods Immunofluorescence staining was performed to analyse the distribution of CD163~+ macrophages and expression of IL-23 and IL-17 in pemphigus vulgaris lesions and healthy human skin tissues. Results The staining of IL-23~+ cells is overlapped with that of CD163~+ macrophages. The mean numbers of IL-23~+ CD163~+ cells and IL-17~+ cells were 80.17 ± 5. 42 and 48. 17 ± 8. 03 in each field of view (×200) in dennis of pemphigus vulgaris, while those in healthy human dermis were 29. 50 ± 4. 45 and 9. 50 ± 3. 35, with significant differences between dennis of pemphigus vulgaris and healthy human dermis (P <0.01). The number of IL23~+ cells was related to that of IL-17~+ cells in dermis (r =0.945, P<0.001). Conclusion IL-23 is mainly secreted by CD163~+ macrophages. IL-23 and IL-17 are overexpressed in pemphigus vulgaris lesions. IL-23/IL-17 cytokine axis may play an important role in the pathogenesis of pemphigus vulgaris.