| 组织工程化表皮膜片修复裸鼠创面的实验研究 |
| |
| 引用本文： | 杨光辉,杨军,邓辰亮,王佳鸣,崔磊,刘伟,曹谊林.组织工程化表皮膜片修复裸鼠创面的实验研究[J].上海交通大学学报(医学版),2004,24(4):293-295. |
| |
| 作者姓名： | 杨光辉 杨军 邓辰亮 王佳鸣 崔磊 刘伟 曹谊林 |
| |
| 作者单位： | 上海第二医科大学第九人民医院整复外科上海市组织工程研究与开发中心,上海200235 |
| |
| 基金项目： | 国家重点基础研究发展规划资助项目(“973”项目)(G1999054309)，国家高技术研究发展计划资助项目(“863”计划)(2001AA216021,2002AA205041)，上海市“重中之重”重点学科基金资助. |
| |
| 摘 要： | 目的 构建组织工程化表皮细胞膜片并应用于修复裸鼠全层皮肤缺损创面。方法 体外分离、培养、扩增人类表皮细胞，与壳聚糖—明胶膜构建表皮细胞膜片，用于修复裸鼠背部全层皮肤缺损创面，以单纯壳聚糖／明胶膜作为对照；术后行大体、组织学观察及免疫组织化学检测。结果 表皮细胞可以壳聚精—明胶膜作为载体在体外培养，达到60％～70％亚融合状态时，移植人表皮细胞／壳聚糖—明胶膜到裸鼠全层皮肤缺损创面，人表皮细胞可以在创面存活并继续增殖分化，形成连续的表皮覆盖创面。结论 人表皮细胞／壳聚糖—明胶膜是一种理想的组织工程表皮替代物。
|
| 关 键 词： | 组织工程 表皮细胞 创面愈合 壳聚糖 明胶膜 皮肤缺损 |
| 文章编号： | 0258-5898(2004)04-0293-03 |
| 修稿时间： | 2003年7月10日 |
Experimental study of tissue engineered epidermal membrane in the repairing of full-thickness skin defects of nude mouse |
| |
| YANG Guang-hui,YANG Jun,DENG Chen-liang,WANG Jia-ming,GUI Lei,LIU Wei,CAO Yi-lin.Experimental study of tissue engineered epidermal membrane in the repairing of full-thickness skin defects of nude mouse[J].Journal of Shanghai Jiaotong University:Medical Science,2004,24(4):293-295. |
| |
| Authors: | YANG Guang-hui YANG Jun DENG Chen-liang WANG Jia-ming GUI Lei LIU Wei CAO Yi-lin |
| |
| Abstract: | Objective To construct tissue engineered epidermal membrane and explore the possibility of repairing the full-thickness skin defects of nude mouse with engineered membrane. Methods Seeded the cultured epidermal cells (EC) on chitosan-gelatin(CG) membrane to form an artificial epidermal grafts. Then the engineered membrane was transplanted to repair the full-thickness skin defects of nude mouse. CG membrane without EC served as control. Results Epidermal cells could be cultured in vitro on CG membrane. When EC reached 60%-70% sub-confluence , the EC-CG membrane was grafted. The human EC could be survived on the wound and continued to proliferate and differentiate to form a layer of new epidermis. Conclusion EC-CG membrane may become an ideal tissue engineered epidermal subsittute. |
| |
| Keywords: | tissue engineering epidermal cells chitosan-gelatin membrane wound healing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
