| 紫杉醇耐药与敏感细胞的差异表达蛋白分析 |
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| 引用本文： | 孙强玲,杨晓华,储天晴,谢银银,鲁静,包国良,冯久贤,龚乐罗,沙慧芳.紫杉醇耐药与敏感细胞的差异表达蛋白分析[J].上海交通大学学报(医学版),2009,29(5). |
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| 作者姓名： | 孙强玲 杨晓华 储天晴 谢银银 鲁静 包国良 冯久贤 龚乐罗 沙慧芳 |
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| 作者单位： | 孙强玲,杨晓华,包国良,冯久贤,龚乐罗,沙慧芳,SUN Qiang-ling,YANG Xiao-hua,BAO Guo-liang,FENG Jiu-xian,GONG Le-luo,SHA Hui-fang(上海交通大学胸科医院基础研究室,200030);储天晴,CHU Tian-qing(上海交通大学胸科医院肺内科,上海,200030);谢银银,鲁静,XIE Yin-yin,LU Jing(医学院瑞金医院,上海血液学研究所,上海,200025)
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| 基金项目： | 上海市市级医院新兴前沿技术联合攻关项目，上海市胸科医院院级课题，上海市慈善基金会癌症研究专项基金 |
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| 摘 要： | 目的 比较人肺腺癌细胞系A549与肺腺癌紫杉醇耐药细胞系A549-Taxol的差异表达蛋白.方法 提取A549和A549-Taxol总蛋白,应用双向凝胶电泳技术(2-DE)分离,ImageMaster 5.0软件分析并筛选出两者间差异表达的蛋白点,质谱分析加以鉴定.结果 获得分辨率高且重复性好的肺腺癌耐紫杉醇细胞系及亲代细胞系2-DE图谱.ImageMaster软件分析差异大于2倍的蛋白质点共有80个,其中33个在耐药细胞系中上调,47个下调.质谱分析鉴定出19种差异表达蛋白,其中A549-Taxol中上调蛋白11种,涉及细胞骨架蛋白、代谢酶类、分子伴侣和信号转导相关蛋白质.结论 应用2-DE技术整体展示了紫杉醇耐药细胞系及敏感细胞系的蛋白表达谱,质谱法鉴定的差异蛋白为进一步从多因素角度研究紫杉醇耐药机制提供了新线索.
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| 关 键 词： | 双向凝胶电泳 蛋白质组学 肺癌 紫杉醇耐药 |
Analysis of differential protein expression profile between paclitaxel resistant and sensitive lung adenocarcinoma cells |
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| SUN Qiang-ling,YANG Xiao-hua,CHU Tian-qing,XIE Yin-yin,LU Jing,BAO Guo-liang,FENG Jiu-xian,GONG Le-luo,SHA Hui-fang.Analysis of differential protein expression profile between paclitaxel resistant and sensitive lung adenocarcinoma cells[J].Journal of Shanghai Jiaotong University:Medical Science,2009,29(5). |
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| Authors: | SUN Qiang-ling YANG Xiao-hua CHU Tian-qing XIE Yin-yin LU Jing BAO Guo-liang FENG Jiu-xian GONG Le-luo SHA Hui-fang |
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| Institution: | 1.Basic Research Laboratory;2.Department of Pulmonary Medicine;Chest Hospital;Shanghai Jiaotong University;Shanghai 200030;China;3.Shanghai Institute of Hematology;Ruijin Hospital;School of Medicine;Shanghai 200025;China |
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| Abstract: | Objective To compare the protein expression profiles between human lung adenocarcinoma cell line A549 and paclitaxel resistant cell line A549-Taxol. Methods Total celluar proteins extracted from A549 and A549-Taxol were separated by two-dimensional polyacrylamide gel electrophoresis(2-DE).High quality 2-DE profiles were obtained and analysed by ImageMaster 5.0 software to screen differentially expressed protein spots.Those spots were identified by mass spectrometry. Results 2-DE patterns of lung adenocarcin... |
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| Keywords: | two-dimensional polyacrylamide gel electrophoresis proteomics lung cancer paclitaxel resistance |
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