苦参碱联合5-FU诱导人胃癌细胞凋亡的机制

目的探讨苦参碱和5-氟尿嘧啶(5-FU)联用诱导人胃癌细胞SGC7901凋亡的机制。方法1.0mg/mL苦参碱联合20mg/L5FU作用于SGC7901细胞48h,RTPCR和免疫细胞化学检测bcl-2、bax、Fas、Fas-L的mRNA和蛋白表达;免疫细胞化学、酶联免疫吸附试验和Westernblot检测活性caspase3的表达,并与空白对照组和单独苦参碱或5-FU用药组比较。结果联合用药组bcl2下调,bax、Fas上调,与空白对照组有显著差异(P<0.05),与单独用药组相比无显著性差异(P>0.05);联合用药组活性caspase-3表达显著增高,与各组比较均有显著差异(P<0.01)。结论苦参碱和5FU联用通过上调bax、下调bcl2/Fax,显著提高活性caspase3的表达,从而协同诱导胃癌细胞SGC7901的凋亡。

Mechanisms Underlying Apoptosis of Gastric Cancer Cell Line Induced by Combination of 5-Fluorouracil and Matrine

Objective To study the mechanism of inducing the apoptosis of gastric cancer cell line SGC-7901 by the combined use of matrine and 5-fluorouracil (5-FU). Methods 1.0 mg/mL of matrine combined with 20 mg/L of 5-FU acted on gastric cancer cell line SGC-7901 for 48 h. Bcl-2, bax, Fas and Fas-L were detected by means of RT-PCR and caspase-3, bcl-2, bax, Fas and Fas-L of activity were detected by means of ELISA and Western blot. Results As compared with the control group, the group of combined drugs was not significantly different with low-regulation of bcl-2 and up-regulation of bax,and Fas (P>0.05) with caspase-3 of active was significantly increased;compared with the control group it was significantly different (P<0.01). Conclusion The combined use of matrine and 5-FU,with up-regulation of Bas and low-regalation of bcl-2/Fax significantly increased the expression of caspase-3 of activity, thereby inducing in coordination the apoptosis of gastric cell line SGC-7901.