| 慢病毒介导BMP2和VEGF165基因共转染对骨髓基质干细胞成骨分化的影响 |
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| 引用本文： | 蒋佳,范存义,曾炳芳.慢病毒介导BMP2和VEGF165基因共转染对骨髓基质干细胞成骨分化的影响[J].上海交通大学学报(医学版),2011,31(6):753-758. |
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| 作者姓名： | 蒋佳 范存义 曾炳芳 |
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| 作者单位： | 1. 复旦大学运动医学中心华山医院运动医学与关节镜外科,上海,200040
2. 上海交通大学附属第六人民医院骨科,上海,200233 |
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| 基金项目： | 上海市科委重大项目(054119520)~~ |
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| 摘 要： | 目的观察慢病毒介导骨形成蛋白2(BMP2)和血管内皮生长因子165(VEGF165)基因共转染对骨髓基质干细胞(MSCs)成骨分化的影响。方法构建携带BMP2、VEGF165和绿色荧光蛋白(GFP)基因的重组慢病毒表达载体,包装、生产携带BMP2、VEGF165和GFP基因的重组慢病毒(Lv-BMP、Lv-VEGF、Lv-GFP)。分离、培养W istar大鼠骨髓MSCs,分别以Lv-GFP(GFP组)、Lv-BMP(BMP组)、Lv-VEGF(VEGF组)转染MSCs,Lv-BMP和Lv-VEGF共转染MSCs(BMP+VEGF组),另设未转染MSCs对照组。RT-PCR法检测转染后7 d各组细胞BMP2、VEGF165 mRNA表达以及转染后1、2、4周各组细胞骨钙素(OCN)mRNA表达;ELISA法检测转染后1、4、8周各组细胞培养上清液BMP2、VEGF165蛋白表达以及转染后1、2、4周各组细胞培养上清液OCN蛋白表达;转染后第14天,各组细胞行碱性磷酸酶(ALP)染色和ALP活性检测。结果 BMP+VEGF组BMP2、VEGF165 mRNA和蛋白高效共表达,BMP2 mRNA和蛋白表达与BMP...
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| 关 键 词： | 骨形成蛋白2 血管内皮生长因子165 共转染 慢病毒载体 成骨分化 |
Effects of lentivirus-mediated co-transfection of BMP2 gene and VEGF165 gene on osteogenic differentiation of bone marrow-derived mesenchymal stromal cells |
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| JIANG Jia,FAN Cun-yi,ZENG Bing-fang.Effects of lentivirus-mediated co-transfection of BMP2 gene and VEGF165 gene on osteogenic differentiation of bone marrow-derived mesenchymal stromal cells[J].Journal of Shanghai Jiaotong University:Medical Science,2011,31(6):753-758. |
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| Authors: | JIANG Jia FAN Cun-yi ZENG Bing-fang |
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| Institution: | JIANG Jia1,FAN Cun-yi2,ZENG Bing-fang2(1.Sports Medicine Center of Fudan University,Department of Sports Medicine and Arthroscopy,Huashan Hospital,Shanghai 200040,China,2.Department of Orthopedics,the Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China) |
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| Abstract: | Objective To investigate the effects of lentivirus-mediated co-transfection of bone morphogenetic protein 2(BMP2) gene and vascular endothelial growth factor 165(VEGF165) gene on osteogenic differentiation of bone marrow-derived mesenchymal stromal cells(MSCs).Methods The expression lentivirus vectors carrying VEGF165,BMP2 or green fluorescent protein(GFP) gene were constructed respectively,and recombinant lentivirus carrying VEGF165(Lv-VEGF),BMP2(Lv-BMP) or GFP(Lv-GFP) were packaged and produced respective... |
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| Keywords: | bone morphogenetic protein 2 vascular endothelial growth factor 165 co-transfection lentiviral vector osteogenic differentiation |
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