胃癌细胞株SGC-7901和MGC-803的细胞周期分布及侵袭能力之比较研究

目的体外培养胃癌细胞株SGC-7901和MGC-803,并比较两种细胞周期分布情况及侵袭能力。方法采用流式细胞术观察两株细胞的周期分布情况;采用涂有Matrigel胶的Transwell小室对胃癌细胞株SGC-7901和MGC-803进行体外侵袭实验,通过检测穿过小室的相对细胞数量比较两株细胞的侵袭能力。结果SGC-7901在G0/G1期、S期、G2/M期的分布百分比分别为(63.74±3.40)%、(30.23±2.70)%、(6.03±0.70)%;MGC-803在G0/G1期、S期、G2/M期的分布百分比分别为(62.94±6.13)%、(28.67±7.76)%、(8.39±1.69)%,两胃癌细胞株对应的周期时相分布百分比差异无统计学意义(P>0.05);检测SGC-7901和MGC-803穿过涂抹有Matrigel胶的聚碳酸酯膜的细胞数分别为65.67±6.03和94.5±3.68,差异具有统计学意义(P<0.05)。结论 MGC-803细胞的侵袭能力明显强于SGC-7901,而两株细胞在对应的周期时相分布情况则无明显差异。

Comparison of the cell cycle and the invasive ability between human gastric cancer cell lines SGC-7901 and MGC-803 in vitro

Objective To culture gastric cancer cell lines SGC-7901 and MGC-803 in vitro,and compare their cell cycle and invasive ability.Methods Cell cycle properties of the cultured cells were evaluated by FCM;in vitro invasion assay using the transwells coating matrigel were performed to detect the invasive ability of gastric cancer cell lines SGC-7901 and MGC-803 by detecting their relative cell amounts of transporting through chamber.Results Distribution percentages of G0/G1 phase,S phase and G2/M phase were(63.74±3.40)%,(30.23±2.70)%,(6.03±0.70)% in SGC-7901cells,and they were(62.94±6.13)%;(28.67±7.76)%,(8.39±1.69)% in MGC-803 cells,there were no significant difference about the distribution percentage of the corresponding cell cycle phases between SGC-7901 and MGC-803(P>0.05);the invasion assay using the transwells coating matrigel in vitro indicated that the invasion percentage of SGC-7901 cells was(65.67±6.03),which was significantly lower than(94.5±3.68) of MGC-803 cells(P<0.05).Conclusions Cell invasive ability of MGC-803 is obviously higher than that of SGC-7901 cells,while there is no significant difference in the distribution percentage of the corresponding cell cycle phases between SGC-7901 and MGC-803.