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辅助性T细胞17和调节性T细胞在哮喘小鼠中的失衡表达及其意义
引用本文:周辰,陈军浩,殷凯生.辅助性T细胞17和调节性T细胞在哮喘小鼠中的失衡表达及其意义[J].中国呼吸与危重监护杂志,2010,9(1):28-31.
作者姓名:周辰  陈军浩  殷凯生
作者单位:1. 江苏省老年医院,江苏省老年医学研究所,江苏南京,210024
2. 南京大学医学院附属鼓楼医院检验中心,江苏南京,210008
3. 南京医科大学第一附属医院呼吸科,江苏南京,210029
基金项目:国家自然科学基金资助项目 
摘    要:目的探讨辅助性T细胞17(Th17)和CD4+ CD25+ Foxp3+调节性T细胞(Treg)的表达失衡在哮喘小鼠发病机制中的作用。方法将24只BALB/c小鼠随机分为哮喘组和正常对照组,每组12只。哮喘组采用卵白蛋白致敏和激发的方法制作小鼠哮喘模型,对照组用等量的生理盐水代替。采用体积描记法测定气道反应性;对BALF中炎性细胞进行计数;流式细胞术检测脾脏单个核细胞中Th17及Treg细胞占CD4+ T细胞比例;ELISA法检测BALF及肺匀浆中Th17及Treg相关细胞因子IL-17和IL-10水平。结果与对照组比较,哮喘组平均气道阻力显著升高(P0.01),Th17细胞比例显著升高(5.68±1.99)%比(2.80±0.82)%,P0.01],而Treg细胞比例显著降低(2.88±0.46)%比(6.10±2.44)%,P0.01],Th17/Treg比值显著升高(1.93±0.41比0.50±0.15,P0.01),BALF及肺匀浆中IL-17水平均显著升高(22.37±3.00)pg/mL比(11.42±2.15)pg/mL,(52.93±5.39)pg/mL比(19.38±2.65)pg/mL,P均0.01],而IL-10水平均显著降低(6.05±1.25)pg/mL比(14.23±2.94)pg/mL,(9.33±1.79)pg/mL比(21.40±2.44)pg/mL,P均0.01]。结论哮喘小鼠Th17细胞数量增多及IL-17水平增高,而Treg细胞数量减少及IL-10水平降低,Th17/Treg细胞比例失衡可能在哮喘的发病机制中具有重要作用。

关 键 词:哮喘  辅助性T细胞17  调节性T细胞  白细胞介素17  白细胞介素10

The Th17/Treg Imbalance in the Pathogenesis of Asthma in a Mouse Model
ZHOU Chen,CHEN Jun-hao,YIN Kai-sheng.The Th17/Treg Imbalance in the Pathogenesis of Asthma in a Mouse Model[J].Chinese Journal of Respiratory and Critical Care Medicine,2010,9(1):28-31.
Authors:ZHOU Chen  CHEN Jun-hao  YIN Kai-sheng
Institution:. ( Department of Pulmonary Medicine, Gerontology Research Institution of Jiangsu Province. Nanjing , Jiaagsu ,210024, China)
Abstract:Objective To investigate the role of T helper 17 (Thl7) cells and CD4^+ CD25^+Foxp3^+ regulatory T cells (Treg) in the pathogenesis of asthma in a mouse model. Methods Twenty-four BALB/c mice were randomly divided into an asthma group and a normal control group,with 12 mice in each group. Asthma model was established by ovalbumin sensitization and aerosol challenge in the asthma group. Airway reactivity was measured by plethysmography. The total and differential cell counts in bronchoalveolar lavage fluid (BALF) were measured. The ratio of Thl7 and Treg cells to mononuclear cells in the spleens of mice were detected by flow cytometry. The levels of IL-17 and IL-10 in BALF and lung homogenates were measured by ELISA. Results The bronchial provocation test showed that the average lung resistance increased remarkably in the asthma group. In spleens of the asthmatic mice, the percentage of Thl7 cells was significantly higher (5. 68 ± 1.99) % vs (2. 80 ± 0. 82) %, P 〈 0. 01 ], and the percentage of Treg cells was significantly lower (2. 88 ±0. d6)% vs (6. 10 ±2. 44)% ,P 〈0. 01 ], with the ratio of Thl7 to Treg significantly increased( 1.93 ±0. 41 vs 0. 50 ±0. 15,P 〈0. 01). In BALF and lung homogenates of the asthma group, the level of IL-17 was significantly higher ( 22. 37 ± 3.130) pg/mL vs ( 11.42 ± 2. 15) pg/mL, ( 52. 93 ± 5. 39 ) pg/mL vs ( 19. 38 ± 2. 65) pg/mL,both P 〈 0. 011, and the level of IL-10 was significantly lower ( 6. 05 ± 1.25 ) pg/mL vs ( 14. 23 ± 2. 94) pg/mL, (9. 33 ± 1.79) pg/mL vs ( 21.40 ± 2. 44) pg/mL, both P 〈 0. 01 ] compared with the control group. Conclusion The imbalance of Thl7/Treg plays an important role in the pathogenesis of asthma.
Keywords:Asthma  T helper 17 cells  Regulatory T cells  Interleukin-17  Interleukin-10
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