慢性阻塞性肺疾病患者血清白细胞介素32水平的变化及意义

目的测定慢性阻塞性肺疾病（COPD）急性加重期和稳定期患者血清白细胞介素32（IL-32）水平,以探讨IL-32在COPD发病机制中的作用。方法 2010年10月至2011年5月收集120例COPD患者,急性加重期60例,稳定期60例。另选同期30例非吸烟健康受试者作为对照组。用ELISA法检测血清IL-32和IL-8、肿瘤坏死因子α（TNF-α）含量,同时测定肺功能（FEV1%pred、FEV1/FVC）,并分析IL-32水平与IL-8、TNF-α和气流阻塞之间的相关性。应用SPSS 13.0软件进行统计学分析,多组数据间比较采用单因素方差分析,采用Pearson相关分析进行相关性检验。结果 COPD急性加重期组血清IL-32的含量（174.56±88.15）ng/L]显著高于对照组（59.41±20.98）ng/L]和COPD稳定期组（89.40±33.84）ng/L]（P均〈0.01）,COPD稳定期组血清IL-32含量显著高于健康对照组（P值〈0.01）;COPD急性加重期组、稳定期组血清IL-32水平与IL-8、TNF-α呈正相关（P均〈0.01）,与FEV1%pred、FEV1/FVC和PaO2呈负相关（P均〈0.01）;不同严重程度COPD患者血清IL-8、TNF-α和IL-32含量比较无统计学意义（P均〉0.05）。结论 COPD患者血清IL-32水平升高,可能与IL-8、TNF-α等炎性因子有关。IL-32可能作为一种促炎因子参与COPD的炎症反应。

The Expression and Implication of Human Interleukin-32 in Serum in Patients with Chronic Obstructive Pulmonary Disease

Objectve To measure the serum levels of human IL-32 and explore the clincal implication in patients with chronic obstructive pulmonary disease(COPD) at acute exacerbation or stable stage.Methods 120 patients with COPD were recruited,including 60 patients with acute exacerbation COPD and 60 patients with stable COPD from October 2010 to May 2011.Thirty healthy nonsmoking volunteers were included as controls.The concentrations of interleukin-8(IL-8),tumor necrosis factor alpha(TNF-α),and IL-32 in serum were measured by enzyme-linked immunosorbent assay(ELISA).The correlations among IL-32,IL-8,TNF-α and lung functions were investigated.The datas were analyzed using a statistical software package(SPSS13.0).Variables were compared with one-way ANOVA.The correlations between variables were analyzed using Pearson’s correlation coefficient or Spearman correlation coefficient.Results Serum IL-32 level was significantly higher in AECOPD patients (174.56±88.15)ng/L] than that in healthy subjects (59.41±20.98)ng/L] and in stable COPD patients (89.40±33.84)ng/L](P<0.05)while serum IL-32 level was also significantly higher in stable COPD patients than in healthy subjects(P<0.05).The serum IL-32 1evel in patients with acute exacerbation COPD and stable COPD was positively correlated with the serum IL-8 level,TNF-α level(respectively P<0.01).The serum IL-32 level was negatively correlated with FEV1/predicted value,FEV1/FVC and PaO2(respectively,P<0.01).There was no statistical significance of the serum IL-32,IL-8 or TNF-α levels in COPD patients with different severity(all P>0.05).Conclusion The serum level of IL-32,a new pro-inflammatory cytokine is elevated in COPD patients,which may be involved in the pathogenesis of inflammation in COPD.