| 乳腺癌患者血中角蛋白mRNA基因的表达 |
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| 引用本文： | 毛秀华,卢华青,张乐鸣,陈晓敏,刘东海,李旭军.乳腺癌患者血中角蛋白mRNA基因的表达[J].现代实用医学,2002,14(3):124-126. |
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| 作者姓名： | 毛秀华 卢华青 张乐鸣 陈晓敏 刘东海 李旭军 |
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| 作者单位： | 1. 315211,宁波大学医学院
2. 宁波市第二医院 |
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| 摘 要： | 目的 分析乳腺癌患者外周血液中角蛋白（CK19）mRNA基因的表达。方法 取20例经病理证实的乳腺癌患者外周血，用Tirizol法提取总RNA，然后进行RT－PCR荧光定量检测，并与正常人群进行对照。结果 正常人群外周血中无CK19表达，20例乳腺癌中有6例阳性表达，总阳性率为29．65％，其中单纯癌阳性率为30．7％（4／13），浸润性导管癌阳性率为28．6％（2／7）。结论 CK19可作为检测外周血循环癌细胞的标志物。
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| 关 键 词： | 乳腺肿瘤 病理学 癌症 角蛋白 血液 mRNA 基因表达 CK19 |
| 文章编号： | 1671-0800(2001)03-0124-03 |
| 修稿时间： | 2001年10月17 |
Expression of cytokeratin 19 mRNA in peripheral blood of patients with breast cancer |
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| MAO Xiu Hua,LU Hua Qing,ZHANG Le Ming,et al..Expression of cytokeratin 19 mRNA in peripheral blood of patients with breast cancer[J].Modern Practical Medicine,2002,14(3):124-126. |
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| Authors: | MAO Xiu Hua LU Hua Qing ZHANG Le Ming |
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| Institution: | MAO Xiu Hua,LU Hua Qing,ZHANG Le Ming,et al.Medical College of Ningbo University,Ningbo 315211,China |
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| Abstract: | Objective To assay the expression of cytokeratin 19 mRNA in peripheral blood of patients with breast cancer. Methods Total RNA in peripheral blood of 20 patients with breast cancer was extracted by Trizol method, and the expression of CK19 mRNA was detected by RT-PCR methed.The results were in contrast to that of 10 normal subjects. Results In the peripheral blood of controls, CK19 was negative. In 20 cases of breast cancer,6 cases were positive. The total positive rate of CK19 was 29.65%.Of these,30.7%(4/13) in simple carcinoma and 28.6%(2/7) in ductal infiltrating carcinoma,respectively. Conclusion CK19 may be a tumor marker for detecting cancer cells in peripheral blood. |
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| Keywords: | Breast tumer/pathology Cancer Keratin/blood |
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