恒磁场对成人骨髓间充质干细胞增殖及细胞周期的影响

目的：观察不同强度恒磁场辐射对成人骨髓间充质干细胞(mesenchymal stemce Hs，MSC)增殖水平及细胞周期的影响。方法：用密度梯度离心法分离成人MSC，再经贴壁筛选法筛选，对生长良好的第3代成人MSC进行恒磁场辐射(强度分别为0．05、0．10、0．50和1．00mT)，连续刺激5天(每天8h)后，用单四唑(MTT)法、流式细胞仪法测定细胞增殖、细胞周期和细胞凋亡情况。结果：0．05mT组细胞增殖显著高于对照组；0．10mT组细胞增殖与对照组相比无明显差异；其余剂量组细胞增殖均显著低于对照组。各组均未发现DNA倍体异常细胞。结论：恒磁场对成人MSC增殖的影响与磁感应强度有关，0．05mT的磁场促进MSC的增殖，0．10mT的磁场对MSC无明显影响，0．50mT和1．00mT的磁场抑制MSC的增殖。

Effect of static magnetic fields on the cellular proliferation and cycle of the Human bone marrow mesenchymal stem cells

Objective: To observe the effect of static magnetic fields(SMF) on the proliferation of bone mesenchymal stem cells(MSC) in human. Methods: The MSC were obtained by using gradient centrifuge method, and then selected by the adhesive method. The third generation cells were irradiated by use of static magnetic fields at different intensities for 5 days(8 h/d). The method of MTT was employed to evaluate the level of proliferation. The parameters regarding the variation of the cell cycle were detected with the flow cytometry(FCM). Results: As compared to the control group, the proliferative rate of the MSC exposed to 0.05 mT SMF was significantly higher; there was no difference between the 0.10 mT group and control group; howere, cell proliferation was attenuated significantly when SMF intensity was 0.50 mT and 1.00 mT. No abnormal ploidy was found in any group. Conclusion: The effect of SMF on the proliferation of MSC is dependent on the magnetic intensity. 0.05 mT SMF can accerate the proliferation of MSC. 0.10 mT SMF have no effects on the growth of MSC. Wherease, 0.50 mT and 1.00 mT SMF can attenuate the growth of MSC.