| 急性淋巴细胞白血病骨髓基质细胞缝隙连接功能的研究 |
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| 引用本文： | 刘耀,张曦,司英健,高蕾,高力,陈幸华. 急性淋巴细胞白血病骨髓基质细胞缝隙连接功能的研究[J]. 医学研究生学报, 2007, 20(4): 343-346 |
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| 作者姓名： | 刘耀 张曦 司英健 高蕾 高力 陈幸华 |
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| 作者单位： | 第三军医大学新桥医院血液科,重庆,400037 |
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| 摘 要： | 目的：研究急性淋巴细胞白血病骨髓基质细胞缝隙连接的功能方法：体外培养正常（正常对照组）及急性淋巴细胞白血病（急淋组）原代骨髓基质细胞,采用细胞免疫组化法及计算机灰度检测观察两组之间Connexin 43表达的变化,采用细胞划痕染料传输技术比较两组之间缝隙连接细胞间通讯（GJIC）功能的差异。结果：急性淋巴细胞白血病骨髓基质细胞Connexin 43的表达较正常骨髓基质细胞明显减低,GJIC功能减弱。结论：急性淋巴细胞白血病骨髓基质细胞缝隙连接功能下降可能与白血病骨髓造血微环境功能异常有关。
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| 关 键 词： | 缝隙连接细胞间通讯 急性淋巴细胞白血病 骨髓基质细胞 |
| 文章编号： | 1008-8199(2007)04-0343-03 |
| 修稿时间： | 2006-09-20 |
Study on communicating function of bone marrow stromal cell of acute lymphocytic leukemia |
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| LIU Yao,ZHANG Xi,SI Ying-jian,GAO Lei,GAO Li,CHEN Xing-hua. Study on communicating function of bone marrow stromal cell of acute lymphocytic leukemia[J]. Bulletin of Medical Postgraduate, 2007, 20(4): 343-346 |
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| Authors: | LIU Yao ZHANG Xi SI Ying-jian GAO Lei GAO Li CHEN Xing-hua |
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| Abstract: | Objective:To study communicating function of bone marrow stromal cell(BMSC)of acute lymphocytic leukemia.Methods:BMSC from normal and patients with acute lymphocytic leukemia were cultured.Connexin 43 expression and gap junction intercellular communication(GJIC) of BMSC was observed by using immunochemistry,computer gray scale detection and dry transfer technique.Results:The Connexin 43 expression in BMSC of acute lymphocytic leukemia is lower than that of normal,GJIC function in acute lymphocytic leukemia is weaken.Conclusion:The decrease of communication function of bone marrow stromal cell form acute lymphocytic leukemia maybe induced the disfunction of hemopoietic microenvironment of leukemia. |
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| Keywords: | Connexin 43 |
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