大鼠脑梗死后室周带神经干细胞增殖和迁移

目的：研究成年大鼠脑梗死后室周带神经干细胞的增殖及迁移。方法：制作大鼠脑梗死模型，将其分为脑梗死后3、5、7和14d组，对照组为假手术组：采用免疫组化法动态检测大鼠脑内5-溴脱氧尿苷嘧啶（BrdU）、双皮质素（DCX）的表达。结果：与对照组相比，室周带BrdU阳性细胞在脑梗死后逐渐增加，并于7d达高峰，14d后开始下降，但仍高于正常水平。梗死后不同时间组室周带BrdU阳性细胞数均高于对照组（P〈0．01）。DCX阳性细胞具有迁移细胞的形态学特征，并向损伤区迁移，于术后5d迁移细胞的数量达高峰。结论：脑梗死可激活室周带神经干细胞原位增殖，并向损伤区迁移。

Generation of neural stem cells in subventricular zone and migration to the ischemic lesion area after cerebral infarction in rats

Objective:To investigate the generation and migration of inherent neural stem cells in the(adult) rats with cerebral infarction. Methods: Rat cerebral infarction model was made and the dynamic expressions of bromodeoxyuridine(BrdU) and doublecortin(DCX) were determined by immunofluorescence staining.Results:Compared with the controls,the number of BrdU immunoreactive cells in the subventricular zone(SVZ) increased significantly(P<0.01) after focal cerebral infarction.The expression of BrdU cells was peaked at day 7,it decreased at day 14 but remained at a level higher than that of the controls.DCX immunoreactive cells in the SVZ were morphologically migrating,they migrated to the ischemic lesion and the number was peaked at day 5(P<0.01). Conclusion:The results indicated that generation of inherent neural stem cells was in the SVZ and the neural stem cells migrated to the ischemic lesion area after ischemic stroke.