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吡格列酮对人脂肪细胞中脂联素及其受体mRNA表达的影响
引用本文:丁亚琴,马向华,沈捷,周建英.吡格列酮对人脂肪细胞中脂联素及其受体mRNA表达的影响[J].医学研究生学报,2008,21(4):360-364.
作者姓名:丁亚琴  马向华  沈捷  周建英
作者单位:1. 南京医科大学第一附属医院内分泌科,江苏南京,210029
2. 南京医科大学第一附属医院基因实验室,江苏南京,210029
基金项目:南京医科大学校科研和教改项目
摘    要:目的:探讨噻唑烷二酮类药物吡格列酮对人脂肪细胞中脂联素及其受体mRNA表达的影响。方法:取外科手术患者腹网膜脂肪组织,进行人前体脂肪细胞的原代培养并诱导其分化。适时用不同浓度的吡格列酮进行药物干预,取分化第10d的脂肪细胞用于实验,分设对照组和药物干预组,提取RNA后以RT-PCR方法检测脂联素及其受体的mRNA的表达,比较其差异。结果:吡格列酮药物干预组人脂肪细胞中脂联素及其受体的mRNA的表达量高于不加药物的对照组。结论:噻唑烷二酮类药物吡格列酮能增加人脂肪细胞中脂联素及其受体的mRNA的表达。

关 键 词:人前体脂肪细胞  脂联素  脂联素受体  吡格列酮
文章编号:1008-8199(2008)04-0360-04
修稿时间:2007年6月22日

Pioglitazone promotes the mRNA expressions of adiponectin and its receptor in human adipocytes
DING Ya-qin,MANG Xiang-hua,SHEN Jie,ZHOU Jian-ying.Pioglitazone promotes the mRNA expressions of adiponectin and its receptor in human adipocytes[J].Bulletin of Medical Postgraduate,2008,21(4):360-364.
Authors:DING Ya-qin  MANG Xiang-hua  SHEN Jie  ZHOU Jian-ying
Abstract:Objective: To explore the effects of thiazolidinediones pioglitazone on the mRNA expressions of adiponectin and its receptor in human adipocytes.Methods: We obtained omental adipose tissue biopsies from patients undergoing elective open-abdominal surgery,performed primary culture and differentiation induction of the human preadipocytes,treated them with pioglitazone at different concentrations,and detected the mRNA expressions of adiponectin and its receptor in them by RT-PCR.Results: The mRNA expressions of adiponectin and its receptor were higher in the pioglitazone groups than in the non-pioglitazone control.Conclusion: Pioglitazone promotes the mRNA expressions of adiponectin and its receptor in human adipocytes.
Keywords:Human preadipocyte  Adiponectin  Adiponectin-receptor  Pioglitazone  RT-PCR
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