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| 含HSVtk基因重组逆转录病毒包装细胞PA317/TK的建立 | |
| 引用本文： | 陈道桢,李淑锋,鲁晓萱,王静,苏宁,刘璐,黄鹰,童冠圣,洪泽辉,张丽珊.含HSVtk基因重组逆转录病毒包装细胞PA317/TK的建立[J].医学研究生学报,2003,16(1):1-3. |
| 作者姓名： | 陈道桢 李淑锋 鲁晓萱 王静 苏宁 刘璐 黄鹰 童冠圣 洪泽辉 张丽珊 |
| 作者单位： | 1. 东南大学医学院,遗传中心,江苏南京,210009 2. 东南大学医学院,病理教研室,江苏南京,210009 3. 东南大学医学院,放免中心,江苏南京,210009 4. 铁道部北京铁路总医院核医学科,北京,100038 |
| 基金项目： | 国家自然科学基金资助课题 (批准号 :3 0 0 70 2 2 9) |
| 摘 要： | 目的 :研究肿瘤的自杀基因疗法 ,建立含单纯疱疹病毒胸苷激酶 (HSVtk)基因重组逆转录病毒包装细胞PA317/TK。 方法 :应用脂质体转移方法将逆转录病毒载体GINaTK导入PA317,经G4 18筛选阳性克隆细胞后分别用PCR和扫描电镜检测HSVtk基因整合和病毒颗粒分泌情况。 结果 :逆转录病毒载体GINaTK导入PA317,G4 18后筛选得到阳性克隆细胞 ,命名为PA317/TK。PCR检测PA317/TK细胞出现一特异性 4 0 4bp阳性条带 ,而PA317细胞没有扩增出相应条带。PA317/TK细胞经扫描电镜检测见其表面有颗粒状突起 ,其周围有许多呈团状的病毒颗粒。证实含HSVtk基因重组逆转录病毒存在于包装细胞中。 结论 :成功建立含HSVtk基因重组逆转录病毒包装细胞 |
| 关 键 词： | 逆转录病毒 包装细胞 单纯疱疹病毒胸苷激酶基因 |
| 文章编号： | 1008-8199(2003)01-0001-03 |
| 修稿时间： | 2002年9月18日 |
Construction of the package cells( PA317/TK) with recombinant retroviral vector containing HSVtk gene |
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| CHEN Dao zhen ,LI Shu feng ,LU Xiao xuan ,WANG Jing ,SU Ning ,LIU Lu ,HUANG Ying ,TONG Guan sheng ,HONG Ze hui ,ZHANG Li shan.Construction of the package cells( PA317/TK) with recombinant retroviral vector containing HSVtk gene[J].Bulletin of Medical Postgraduate,2003,16(1):1-3. | |
| Authors: | CHEN Dao zhen LI Shu feng LU Xiao xuan WANG Jing SU Ning LIU Lu HUANG Ying TONG Guan sheng HONG Ze hui ZHANG Li shan |
| Institution: | CHEN Dao zhen 1,LI Shu feng 1,LU Xiao xuan 1,WANG Jing 1,SU Ning 2,LIU Lu 3,HUANG Ying 3,TONG Guan sheng 4,HONG Ze hui 1,ZHANG Li shan 1 |
| Abstract: | Objectives:In order to study suicide gene therapy on tumor, we constructed the package cell PA317/TK with recombinant retroviral vector containing HSVtk gene. Methods:GINaTK retroviral vector containing HSVtk gene was transduced into PA317 retroviral packaging cell by lipofectin,PA317 cells transfected with HSVtk gene were then screened by G418 medium,and positive clones were respectively measured with PCR and with scan electron microscrope. Results: PA317 cells were transfected successfully with HSVtk gene by lipofectin(named PA317/TK).HSVtk gene integrated into PA317 cells was further identified with PCR.Package cells contain recombinant retrovirus particulate with HSVtk gene with scan electron microscrope. Conclusions: The package cells( PA317/TK) with recombinant retroviral vector containing HSVtk gene were successfully constructed. |
| Keywords: | Retrovirus Package cell HSVtk gene |
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