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生物降解性骨组织工程支架材料-可吸收性珊瑚羟基磷灰石
引用本文:曹罡,陶凯,毛天球,曹军,郭婷,陆斌,席庆.生物降解性骨组织工程支架材料-可吸收性珊瑚羟基磷灰石[J].医学研究生学报,2005,18(3):212-216.
作者姓名:曹罡  陶凯  毛天球  曹军  郭婷  陆斌  席庆
作者单位:1. 南京军区南京总医院口腔科,江苏南京,210002
2. 第四军医大学附属秦都口腔医院颌面外科,陕西西安,710033
3. 第四军医大学附属秦都口腔医院正畸科,陕西西安,710033
摘    要:目的:比较可吸收性珊瑚羟基磷灰石(BCHA) Interpore 500R、珊瑚羟基磷灰石(CHA) Interpore 500及天然角孔珊瑚(NGC)复合成骨细胞后,在动物体内形成新骨数量和质量上的差异,对它们作为骨组织工程支架材料的优劣做出评价。方法:应用x一射线衍射分析仪对孔径为500μm的Interpore 500R、Interpore 500及NGC的组成成分进行比较,将三种材料制成标准大小的薄片,植入裸鼠体内,测定不同时间点的降解率。同时将生长状态良好的成骨细胞接种于三种支架材料上,体外孵育4~6h后植入裸鼠背部皮下,分别于术后30、60和90天取材,测量其体积变化,并进行组织学观察,利用图像分析系统测定三种残余材料与形成新骨的面积百分比。结果:Interpore 500R的主要化合物成分为90%文石 10%羟基磷灰石(HA)。它具有高孔隙率,孔隙大小分布均匀,孔隙间交通好。Interpore 500R的生物降解率介于Interpore 500和NGC之间,但从组织工程骨形成的数量、质量上来看均高于其他两种。结论:BCHA作为骨组织工程支架材料明显优于CHA和天然珊瑚(NC),是目前较为理想的骨组织工程支架材料。

关 键 词:生物降解  羟基磷灰石  珊瑚    成骨细胞
文章编号:1008-8199(2005)03-0212-03
修稿时间:2004年6月25日

A new tissue engineering scaffold-biodegradable coral-hydroxyapatite
CAO Gang,TAO Kai,MAO Tian-qiu,CAO Jun,GUO Ting,LU Bin,XI Qing.A new tissue engineering scaffold-biodegradable coral-hydroxyapatite[J].Bulletin of Medical Postgraduate,2005,18(3):212-216.
Authors:CAO Gang  TAO Kai  MAO Tian-qiu  CAO Jun  GUO Ting  LU Bin  XI Qing
Institution:CAO Gang1,TAO Kai2,MAO Tian-qiu2,CAO Jun3,GUO Ting1,LU Bin2,XI Qing2
Abstract:Objective: To Compare the component of biodegradable coral-hydroxyapatite(BCHA) Interpore 500R,coral-hydroxyapatite(CHA) Interpore 500 and nature goniopora coral (NGC). Then using them as cell culturing scaffold observed the differences of tissue engineering(TE) bone formation in vivo of nude mice. Methods: Using X-ray diffraction to analyzed the component of Interpore 500R, Interpore500 and NGC. Surveyed their degradation rates in nude mice. Inoculated the osteoblasts on them for 4-6 h.Implanted the cells/ scaffolds into the back of nude mice. 30 d ,60 d,90 d after implantation we observed the formed TE bone using HE dye.Then using the image-analysis system assayed the percentage of each material remnant and new formed bone. Results: Interpore 500R was mixture of aragonite and hydroxyapatite. The proportion of calcium carbonate in it was 90%. Its biodegradation rate was 50% for three months in vivo of nude mice. And Interpore 500 was 14%, NGC 90%. The newly formed TE bone of Interpore 500R was excellent at quantity and quality comparing to Interpore 500 and NGC. Conclusion: Interpore 500R was an fairly ideal scaffold for bone TE.
Keywords:Biodegradation  Hydroxyapatite  Nature coral  Bone  Osteoblast
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