首页 | 本学科首页   官方微博 | 高级检索  
检索        

纯化抗原半定量检测幽门螺杆菌感染
引用本文:路又可,许国铭,周志群,张洪富.纯化抗原半定量检测幽门螺杆菌感染[J].医学研究生学报,1997(1).
作者姓名:路又可  许国铭  周志群  张洪富
作者单位:南京军区南京总医院消化科!(南京,210002),第二军医大学长海医院消化内科,第二军医大学长海医院消化内科,第二军医大学长海医院消化内科
摘    要:目的 :鉴定并提取幽门螺杆菌 ( Hp)特异性抗原 ,建立测定抗 Hp抗体的半定量免疫酶技术。  方法 :10株 Hp可溶性蛋白用于十二烷基磺酸钠 -聚丙烯酰胺凝胶电泳和免疫印迹分析 ,凝胶层析提取特异性抗原 ,方阵滴定建立免疫酶方法。纯化抗原与全菌抗原同批检测 136例患者 ,以免疫印迹分析作为金标准。  结果 :Hp主要特异性抗原分子量为 660 0 0 ,590 0 0 ,310 0 0和2 50 0 0 ,Sephadex G- 2 0 0柱层析第 1峰提取物含其中 3种抗原成分。纯化抗原 EL ISA测定抗 HpIg G,敏感性 98.9% ,特异性 92 .5% ,准确性 97.0 % ,优于应用全菌抗原的检测结果。  结论 :本实验建立的检测抗 Hp Ig G方法 ,具有敏感、特异、半定量可靠、可目测定性及可用于微量检测等特点 ,值得推广应用

关 键 词:幽门螺杆菌  免疫印迹分析  凝胶色谱层析  酶联免疫吸附测定

Purification of the specific antigens of Helicobacter pylori(Hp) for semiquatitative serodiagnosis.
Lu Youke,Xu Guoming,Zhou Zhiqun etal.Purification of the specific antigens of Helicobacter pylori(Hp) for semiquatitative serodiagnosis.[J].Bulletin of Medical Postgraduate,1997(1).
Authors:Lu Youke  Xu Guoming  Zhou Zhiqun etal
Abstract:Objectives:To analyse and extractthe specific antigens of Hp which were to be used in the establishment of a new immunoenzyme technique. Methods:Hp specific antigens were analysed with SDS- PAGE and Western blot on dissoluble proteins from ten strains,extracted with square titration for the detection of anti- Hp Ig G by ELISA.One hun- dred and thirty- six serum samples were examined with the specific antigens and whole- cell antigens of Hp simultaneously,immunoblot was used as a"gold standard". Results:The major immunogenic polypeptides of Hp were the bands at6 6 0 0 0 ,5 90 0 0 ,31 0 0 0 and2 5 0 0 0 , out of which three antigens were extracted.The sensitivity,specificity and accuracy of the new method was 98.9% ,92 .5 % and 97.0 % respectively. Conclusions:Depurantantigens were superior to whole- cell antigen in measuring anti- Hp Ig G.
Keywords:Helicobacter pylori  Western blot  Gel chromatograph  Enzyme linked immunosorbent assay
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号