塞来昔布联合多柔比星（ADM）对人乳腺癌细胞MCF-7增殖的影响

目的初步探讨塞来昔布联合多柔比星（ADM）对人乳腺癌细胞MCF-7的增殖和凋亡的影响，及其可能机制。方法用不同浓度的塞来昔布溶液、ADM溶液及二者联合用药（简称3药）分别与MCF-7细胞共育24h、48h、72h后，MTT法测定其对细胞的抑制作用。分别用3药作用MCF-7细胞48h后，流式细胞仪检测细胞周期和凋亡。结果10mg／L多柔比星、50μmol／L塞来昔布溶液分别与MCF-7细胞共育24h后，对MCF一7细胞的生长均有明显抑制作用（P〈0．01）。联合用药组的抑制作用更为显著。流式细胞术检测发现，给药细胞48h后，G0／G1期细胞比例升高，S期和G2／M期比例下降，3个给药组细胞均形成明显的凋亡峰，实验组各组与对照组的凋亡率差异有统计学意义（P〈0．01）。结论①塞来昔布和多柔比星均有抑制乳腺癌MCF-7细胞的作用，并呈时间和剂量依赖性；②塞来昔布能诱导MCF-7细胞凋亡，可能与阻止细胞周期进展有关；③塞来昔布与多柔比星联合应用可起到协同抗肿瘤的作用。

Effect of celecoxib and adriamycin on proliferation of breast cancer cell line MCF-7

Objective To study the roles of celecoxib and adriamycin in the inhibition of proliferation and the induction of apoptosis human breast cancer cells MCF-7. Methods MCF-7 cells together with celecoxib or/and adriamycin of different concentration after fostering 24、48、72 hours were carried out, the inhibition of proliferation was measured by MTT assay. Cell cycle and apoptotic percentage were analyzed by flow cytometry(FCM). Results MCF-7 cells together with adriamycin (10mg/L) after fostering 24 hours were pressed down obviously(P<0.01), MCF-7 cells together with celecoxib(50μmol /L) after fostering 24 hours were pressed down obviously(P<0.01), the effect was more obviously in the group of celecoxib combined and ADM. The flow cytometry shows that the proportion of G0/G1 phase and the apoptotic rate wee incresed significantly in all groups, the apoptotic rate was significant between all groups and control group(P<0.01). Conclusions The inhibition of celecoxib and adriamycin against human breast cancer MCF-7 is obviously with time dependence and dose dependence. Celecoxib can induce the apoptosis of human breast cancer MCF-7. Anti-tumor action of celecoxib combined and adriamycin against human breast cancer is cooperated.