一个多发性内分泌腺瘤病2型家系的RET 基因检测 |
| |
引用本文: | 龚莉琳,田波,李蓉,汪志红,冯正平,程庆丰,张素华,李启富.一个多发性内分泌腺瘤病2型家系的RET 基因检测[J].内分泌外科杂志,2014(6):482-485. |
| |
作者姓名: | 龚莉琳 田波 李蓉 汪志红 冯正平 程庆丰 张素华 李启富 |
| |
作者单位: | 400016,重庆医科大学附属第一医院内分泌科 |
| |
基金项目: | 国家临床重点专科建设项目经费资助 |
| |
摘 要: | 目的:对一个多发性内分泌腺瘤病2型( multiple endocrine neoplasia 2,MEN2)家系进行RET基因检测,明确诊断和分型,指导治疗、预防及改善预后。方法采用聚合酶链反应和直接基因测序的方法,对一个临床诊断MEN2的家系(共3名家系成员)和3例正常对照的RET原癌基因21个外显子进行测序。结果该家系中的2例患者和1例无症状一级亲属均为RET原癌基因外显子11第634号密码子位点的杂合错义突变TGC→CGC,半胱氨酸→精氨酸( C634R)。结论通过RET基因检测明确该家系为多发性内分泌腺瘤病2A型,对家系中患者的治疗和随访起到了指导作用,并筛查出家系中无症状的基因突变携带者。
|
关 键 词: | 多发性内分泌腺瘤病 原癌基因 基因检测 |
Gene test of RET-proto-oncogene in a Chinese family of multiple endocrine neoplasia type 2 |
| |
Authors: | Gong Lilin Tian Bo Li Rong Wang Zhihong Feng Zhengping Cheng Qingfeng Zhang Suhua Li Qifu |
| |
Institution: | ( Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China) |
| |
Abstract: | Objective To test the RET-proto-oncogene in a multiple endocrine neoplasia type 2( MEN2) family for confirming the diagnosis and classification , guiding treatment and prevention , and improving the prog-nosis.Methods There were 2 patients of MEN2 with clinical diagnosis and 1 asymptomatic first-degree relative in the pedigree .PCR and direct gene sequencing of PCR produces were used to scan the entire 21 exons of RET-proto-oncogene in the 3 members of the pedigree and 3 normal controls .Results The 2 patients and 1 asympto-matic first-degree relative in the pedigree all had a mutation of the codon 634 in exon 11.It was a heterozygous missense mutation C634R(TGC → CGC).The 3 normal controls showed no abnormalities .Conclusion The gene test of RET proto-oncogene helps to confirm the diagnosis of the pedigree as MEN 2, which can guide the treatment and help identify one asymptomatic mutation carrier . |
| |
Keywords: | Multiple endocrine neoplasia Proto-oncogene Gene test |
本文献已被 维普 万方数据 等数据库收录! |