大鼠荷子宫肌瘤组织移植瘤模型的建立

目的 通过对应用免疫抑制剂的大鼠进行组织移植的方法,建立大鼠荷子宫肌瘤组织移植瘤模型,为研究子宫肌瘤的研究提供新的动物模型?方法 术前两周开始,大鼠予免疫抑制剂吗替麦考酚酯(mycophenolate mofetil,MMF)灌胃,持续至整个实验结束?将20 只Wistar 大鼠随机分为4 组,按照腹腔移植术后补充的不同激素分别为A 组(芬吗通)?B 组(雌二醇)?C 组(达芙通)?D 组(对照组),术后4 周取材,观察肌瘤形态及组织形态学?结果 A 组造模成功率90%,B 组造模成功率40%,C 组?D 组造模成功率为0?结论 大鼠荷子宫肌瘤移植瘤模型成功率高,成本低,可作为新的研究子宫肌瘤移植瘤的动物模型?

Establishment of a rat xenograft model of human uterine leiomyoma

Objective To establish a rat xenograft model of human uterine leiomyoma using immunosuppressive agent and provide a useful tool for the study on uterine leiomyoma. Methods Intragastric administration with immunosuppressive agent mycophenolate mofetil (MMF) (40 mg/ kg) was given to rats for two weeks before the surgery until the end of the experiment. 20 SPF female Wistar rats were randomly divided into 4 groups after abdominal transplantation of human leiomyoma tissues: group A received femoston containing 0.4 mg/ kg estradiol and 2 mg/ kg dydrogesterone, group B received estadiol 0.4 mg/ kg, group C received dydrogesterone 2 mg/ kg, and group D served as the control group, received distilled water 1 mL/200 g. All rat received the corresponding drugs once per day for 2 days. Samples were taken at 4 weeks after the surgery to observe the pathology of the tumor tissues. Results The modeling success rates were 90% in the group A, 40 % in the group B, and 0% in the groups C and D. Conclusions Rat xenograft model of human leiomyoma can be successfully established using an immunosuppressive agent femostone with a high modeling success rate and low cost. It can be used as a new animal model for the study of transplanted leiomyoma.