利用多重荧光STR技术分析上海地区7品系常用近交系小鼠核心群的遗传特性

目的 建立一种裸鼹鼠骨髓巨噬细胞分离培养的方法,并通过与小鼠骨髓巨噬细胞进行比较研究其吞噬功能.方法 分离裸鼹鼠骨髓细胞,在含60 ng/mL巨噬细胞集落刺激因子(M-CSF)完全培养基的诱导下,体外贴壁培养6～7 d,采用倒置显微镜观察细胞的生长状态.裸鼹鼠骨髓细胞诱导结束后,采用流式细胞术检测贴壁细胞表面抗原CD11b和F4/80的阳性率,鉴定骨髓巨噬细胞的纯度;采用异硫氰酸荧光素(FITC)-dextran根据荧光强度比较裸鼹鼠和ICR小鼠骨髓巨噬细胞的吞噬功能.结果 通过本方法获得的贴壁细胞具有典型的巨噬细胞的形态特征,且细胞表面抗原CD1 1b和F4/80的阳性率均高于80％;裸鼹鼠与ICR小鼠骨髓巨噬细胞的吞噬率分别为99.87％士0.13％和88.79％±0.90％.结论 本文建立的方法是一种简单实用的体外分离培养裸鼹鼠骨髓巨噬细胞的方法,且能够获得高纯度、高活性的巨噬细胞.裸鼹鼠骨髓巨噬细胞的吞噬功能高于小鼠骨髓巨噬细胞.

Genetic characteristics of seven inbred mouse strains in core colonies from Shanghai analyzed using fluorescent multiplex STR

Objective To analyze the genetic characteristics of seven frequently-used inbred mouse strains in core colonies from Shanghai suppliers. Methods 48 pairs of microsatellite primers with high polymorphism were screened out and combined into 11 sets of multiplex PCR primer mix labelled with two dyes (FAM and HEX). By these primer mix, the DNA samples of the seven strains were genotyped. The genotyping data were analyzed using statistical software. Results All the 48 microsatellite loci were homozygous for all inbred mice of the seven strains. On each microsatellite locus, the PCR results of the mice in the same population showed monomorphism, and that of the mice in different populations showed polymorphism, whether the populations were of the same strain or not. But the genetic distances between the populations of the same strain were all closer than that of different strains. The phylogenic tree showed that the populations of the same strain were first get into one cluster. The genetic relationship between the strains of C57BL/6 and other six strains were all far. Conclusions Genetic differences exist on 48 microsatellite loci between the core colonies of seven inbred mouse strains from two suppliers in Shanghai.