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miR-200b-3p和miR-200b-5p在马立克氏病抗性与易感SPF鸡法氏囊组织的差异表达分析
引用本文:王瑞琪,廉传江,易诚,韩凌霞,杨春文,陈洪岩.miR-200b-3p和miR-200b-5p在马立克氏病抗性与易感SPF鸡法氏囊组织的差异表达分析[J].实验动物与比较医学,2017(3):244-248.
作者姓名:王瑞琪  廉传江  易诚  韩凌霞  杨春文  陈洪岩
作者单位:1. 牡丹江师范学院生命科学与技术学院,牡丹江157011;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/黑龙江省实验动物与比较医学重点实验室,实验动物与比较医学创新团队,哈尔滨150069;2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/黑龙江省实验动物与比较医学重点实验室,实验动物与比较医学创新团队,哈尔滨150069;3. 牡丹江师范学院生命科学与技术学院,牡丹江,157011
基金项目:国家自然科学基金项目(31500997),黑龙江省科技计划项目(PC13S04)
摘    要:目的 在前期高通量测序分析的基础上,进一步利用荧光定量PCR验证miR-200b-3p和miR-200b-5p在马立克氏病(MD)抗性(B21单倍型)与易感(B19单倍型)鸡法氏囊组织的差异表达情况.方法 通过经优化后确定的最佳实时荧光定量PCR反应体系和扩增条件,进行荧光定量PCR分析.结果 miR-200b-3p和miR-200b-5p荧光定量PCR结果与高通量测序结果上下调情况基本一致,但差异表达显著性分析结果却不完全相同.结论 尽管差异表达显著性分析结果不完全一致,但两种方法的结果均提示来源于同一前体的miR-200b-3p和miR-200b-5p可能与MD抗性/易感性相关.

关 键 词:miR-200b  马立克氏病(MD)  抗性/易感性  高通量测序  荧光定量PCR

Differential Expression Analysis of miR-200b-3p and miR-200b-5p in Marek's Disease Resistant and Susceptible SPF Chickens
WANG Rui-qi,LIAN Chuan-jiang,YI Cheng,HAN Ling-xia,YANG Chun-wen,CHEN Hong-yan.Differential Expression Analysis of miR-200b-3p and miR-200b-5p in Marek's Disease Resistant and Susceptible SPF Chickens[J].Laboratory Animal and Comparative Medicine,2017(3):244-248.
Authors:WANG Rui-qi  LIAN Chuan-jiang  YI Cheng  HAN Ling-xia  YANG Chun-wen  CHEN Hong-yan
Abstract:Objective To validate high throughput sequencing results by fluorescence quantitative PCR technique and analyze the expression of miR-200b-3p and miR-200b-5p in bursa,and explore possible molecular mechanism associated with Marek's disease (MD) resistance/susceptibility.Methods Through optimization,the ideal amplification conditions and reaction system of real-time fluorescence quantitative PCR were identified.Results miR-200b-3p and miR-200b-5p fluorescence quantitative PCR results and high throughput sequencing results were consistent in down-regulation,but not significant difference.miR-200b-3p and miR-200b-5p two homologous miRNA showed similar expression after infected by Marek's disease virus (MDV).Conclusion Although the difference is not consistent with the results of significant analysis,miR-200b-3p and miR-200b-5p may be associated with resistance / susceptibility of MD.
Keywords:MiR-200b  Marek's disease (MD)  Resistance/susceptibility  High throughput sequencing  Fluorescence quantitative PCR
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