大鼠视网膜色素上皮细胞分离的改良及其MMP表达

目的观察束缚应激致亚健康状态大鼠模型的免疫功能的改变，探讨中药的干预作用及其机制。方法40只雄性SD大鼠，随机分为正常对照组、模型对照组、抗衰老片组和六味地黄丸组，采用限制活动模拟人类心理应激因素，建立大鼠亚健康状态模型，同时，用抗衰老片和六味地黄丸进行干预，测定应激大鼠的免疫功能指标。结果造模21d后，模型大鼠的T淋巴细胞增殖能力、NK细胞活性、IL-2和IFN-γ含量及胸腺指数均显著降低（P〈0．05）。给予抗衰老片和六味地黄丸后，模型大鼠T、B淋巴细胞增殖能力、胸腺指数、IL-2含量均显著升高俨〈0．05），NK细胞活性有升高趋势。结论束缚应激致亚健康状态大鼠模型的免疫功能下降，给予抗衰老片和六味地黄丸后，可明显提高亚健康状态大鼠的免疫功能，提示中药提高免疫功能的作用可能与调节机体细胞与体液免疫有关。

A Modified Method of Isolation of Rat Retinal Pigment Epithelial Cells and the Expression of Matrix Metalloproteinases in the Cultured Cells

Objective To observe the changes of immune function in sub-health state rat model induced by restraint stress and to explore the mechanism of the traditional Chinese medicine alters the stress. Methods Forty Male SD rats were randomly divided into 4 groups： normal control group, model control group, anti-ageing tablets group and Liuwei Dihuang pills group. To simulate human physiological stress, the sub-health state rat model was induced by restraint. The rats in administration group were given anti-aging tablets and Liuwei Dihuang pills respectively during the model establishment process. At the end of experiment, the indexes of immune function of sub-health state rats tested. Results Twenty-one days after the experiment, T lymphocyte proliferation capacity, NK cell activity, content of serum IL-2, IFN-T and thymus index decreased significantly （P〈0.05）. After treatment of anti-aging tablets and Liuwei Dihuang pills, T and B lymphocyte proliferation capacity, thymus index and content of serum IL-2 were significantly increased （P〈0.05）, NK cell activity had an increasing trend. Conclusion The immune function decreased in sub-health state rat model induced by restraint stress, and anti-ageing tablets and Liuwei Dihuang pills could improve the immune function of sub-health state rats significantly.The result suggested that the effect of improving immune function by traditional Chinese medicine may be related to regulate cellular and humoral immunity.