颈动脉负压分流制作大鼠全脑缺血/再灌注模型

为观察被动吸烟大鼠肺泡灌洗液炎性细胞及呼吸道病理形态学改变,将清洁级SD大鼠随机分成两组,吸烟组(n=13)置于隔离器内被动吸烟2 h/d,共20 d,对照组置隔离器内2 h/d,无被动吸烟.20 d后行支气管肺泡灌洗液(BALF)炎性细胞计数及分类,并作右总支气管气道表面电镜下的病理形态学观察及肺组织学检查.结果显示吸烟组大鼠BALF中炎性细胞计数为(46.1±28.3)×106/L,较对照组(17.7±9.9)×106/L明显增高(P《0.05).光镜检查示吸烟组小气道和肺组织炎性细胞浸润,扫描电镜观察见柱状上皮细胞纤毛倒伏凌乱,部分脱落;对照组未见明显异常.烟雾刺激导致大鼠呼吸道炎性细胞增多,炎症反应增强及纤毛功能减退,因而被动吸烟可能是引起呼吸道损伤的重要因素.

A Novel Model of Cerebral Ischemia/reperfusion Indu ced by Carotid Artery Negative Pressure Shunt in Rats

Objective To establish a new model of whole cerebral ischemia/reperfusion in rats on basis of anatomic and hemodynamic principle Methods Whole cerebral ischemia in rats was induced by clamping the bilateral common carotid arteries and drawing some blood from right common carotid artery continuously through right external carotid artery Meanwhile the drawn blood was retransfused into left femoral vein Reperfusion began after stopping drawing blood and removing the artery clamps Electroencephalograph(EEG),light microscope(LM) and electron microscope(EM) were used to assess the result of ischemia Results Compared with control group,the whole cerebral in the experimental group showed obvious ischemia changes in EEG,LM and EM Conclusion This novel model has several advantages such as the reliable whole cerebral ischemia,sufficient reperfusion,high rate of success,fit for injecting reagent through carotid artery,and is useful to the study of whole cerebral ischemia/reperfusion