白细胞介素－2受体表达在近交系小鼠遗传监测中应用的研究

采用免疫组织化学方法，通过检测骨组织移植后宿主淋巴细胞白细胞介素－２受体（ＩＬ－２Ｒ）的表达，判定近交系小鼠Ｈ－２基因的纯合性，以期作为近交系小鼠遗传监测的方法。选用Ｃ５７ＢＬ／６系小鼠，实验前进行了标准的组织相容性基因检测（皮肤移植法），观察期为５０ｄ，每１０ｄ为一个观察点。结果显示，与皮肤移植结果一致。该方法似可作为近交系小鼠的遗传监测的手段之一。

The Application of Interleukin-2 Receptor Expression Assay on the Genetic Monitoring of Inbred Strain Mice

he aim of this study was to evaluate the mouse H-2 genetic loci of inbred strains by analysing the expression rate of host sensitive lymphocyte interleukin-2 receptor with immunohistochemistry method. C 57BL/6 male adult mice were used as animal model in the experiment. Standard assay of histocompatibility(skin grafts)in the mice had been finished before, in which fresh allogenic bone (from DBA/2)and isogenic bone (from C57 BL/6) were transplanted. The experiment results were comparable to the standard assay for evaluating the histocompatibility of the inbred strain of mouse.The results suggested that the interleukin-2 receptor expression assay can be used as one of the methods for the genetic monitoring of inbred mice.