| 5-氮胞苷对贵州小型猪淋巴细胞DNA损伤及修复的影响 |
| |
| 引用本文： | 钱宁,李达圣,甘世祥.5-氮胞苷对贵州小型猪淋巴细胞DNA损伤及修复的影响[J].实验动物与比较医学,2002,22(4):223-226. |
| |
| 作者姓名： | 钱宁 李达圣 甘世祥 |
| |
| 作者单位： | 1. 福建医科大学,分子医学研究中心,福州,350004
2. 福建医科大学,附属协和医院,福州,350004 |
| |
| 摘 要： | 应用组织块静置培养法建立成年水牛晶状体上皮细胞的离体培养方法,通过细胞计数法、MTT法和透射电镜法观察,对晶状体上皮细胞的离体培养和冻存复苏的可行性进行研究,结果显示离体培养和冻存复苏后的晶状体上皮细胞生长良好且二者无显著差异(P>0.05),并保持了原有上皮细胞的特性及细胞结构。提示组织块静置培养法是适宜的晶状体上皮细胞培养方法,液氮冷冻可有效地保存晶状体上皮细胞。
|
| 关 键 词： | 晶状体上皮细胞 培养 冻存 复苏 超微结构 |
| 文章编号： | 1004-8448(2002)04-0223-04 |
| 修稿时间： | 2001年2月1日 |
Study of DNA Damage and Repair Induced by 5-Azacytidine in Lymphocytes of Guizhou Miniature Pig |
| |
| QIAN Ning,LI Dasheng,GAN Shixiang.Study of DNA Damage and Repair Induced by 5-Azacytidine in Lymphocytes of Guizhou Miniature Pig[J].Laboratory Animal and Comparative Medicine,2002,22(4):223-226. |
| |
| Authors: | QIAN Ning LI Dasheng GAN Shixiang |
| |
| Abstract: | The modified method for cultivating an adult buffalo lens epithelial cells was described. The cell growth in vitro and the recovery of cells from frozen stale was investigated by cell counting ?MTT and transmission electron microscopy. The results appeared that there is no significant difference in growth between cells cultured in vitro and recovered from frozen stale (P>0. 05), with characteristic and structure similar to the normal epithelial cell. The results suggested that planting culture is a kind of culture method for lens epithelial cells and freezing is an effective method to keep in cryopreser-vation such cells. |
| |
| Keywords: | Lens epithelial cells Culture Cryopreservation Recovery Ultrastructure |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
| 点击此处可从《实验动物与比较医学》下载免费的PDF全文 |
