H7N9禽流感病毒小鼠感染动物模型的建立

目的 建立H7N9小鼠感染动物模型。方法 1?08，1?07或1?06 TCID50H7N9禽流感病毒原液（A/Anhui/1/2013）滴鼻感染BALB/c小鼠。主要观测指标：临床症状、死亡率、病理变化、病毒载量和血清抗体检测。结果 被感染的小鼠表现为竖毛、弓背、体重下降；病理表现为间质性肺炎，感染后第2天开始在呼吸道脱落细胞中检测到病毒；免疫组化或病毒分离方法在肺、肾、脑、肠、脾等组织检测到病毒；感染后14d在小鼠血清中血凝抑制试验特异性抗体效价达到160；淋巴细胞减少，单核细胞增多。结论 H7N9感染BALB/c小鼠模型与人类禽流感感染疾病的基本特征相似，为研究该病的发病机制及药物疫苗的研发提供了工作基础。

The Mouse models for the Novel Avian-Origin Human Influenza A (H7N9) Virus

Objective The current study was conducted to establish mouse model for the novel avian-origin H7N9 influenza virus. Methods A/Anhui/1/2013 (H7N9) virus was administered by intranasal instillation to groups of BALB/c mice. The changes of body weight and clinical signs were observed every 24h from 1 to 14d. Blood and several organ samples were taken. Pathologic changes were observed, and avian influenza A (H7N9) virus was detected with virus titrations and immunohistochemistery(IHC).Results Mice developed typical clinical signs including body weight loss, ruffled fur and death. Peak virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.). Virus could also be detected in brain, liver, spleen, kidney and intestine from inoculated mice. The inoculation of H7N9 could elicit seroconversion titers up to 160. Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed. Conclusion The mouse enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.