代谢性疾病实验动物模型的建立与评价

目的观察不同性别、不同年龄的WHBE兔血气、电解质及能量代谢的变化。方法取幼年、青年、老年WHBE兔经耳中动脉取肝素抗凝血，在NOVA血气电解质分析仪上测定血气、电解质指标。结果（1）幼年雄性WHBE兔血糖（Glu）明显低于雌性（P〈0．05），青年雄性WHBE兔SO2、Hb和R均显著高于雌性（P〈0．05），老年WHBE兔性别间无明显差异（P〉0．05）；（2）青年WHBE兔PCO2、BE-ECF、BE—B、SBC、HC03^-、TCO2、以均明显高于幼年职0．05，P〈0．01），而PO2、SO2、O2Ct、A和PO2／FI均明显低于幼年（P〈0．05，P〈0．01）；老年WHBE兔血液pH、PCO2、BE—ECF、BE—B、SBC、HCO3^-、TCO2、a／A和Osm均明显高于幼青年（P〈0．05，P〈0．01），而PO2、SO2、Het、Hb、O2Ct、A和PO2／FI均明显低于幼青年限0．05，P〈0．01）。结论性别因素对幼年WHBE兔Glu、青年WHBE兔SO2、Hb和K^＋有影响，而年龄对WHBE兔的血气、电解质和能量代谢影响随年龄增长而变化，与WHBE兔的生理生长密切相关。

Establishment and Evaluation of Laboratory Animal Models of Metabolic Diseases

Objective To observe the changes of blood gas, electrolytes and energy metabolism in WHBE rabbits among different ages. Methods One hundred and thirty-three WHBE rabbits were divided into 3 groups, infant, young or senile groups respectively, the blood sample were taken from center artery in ear, and the blood gas and electrolytes indexes were detected by NOVA blood gas and electrolytes analyzers. Results （1） Effect of gender, the Glu index was lower in male infant rabbits than that of female ones（P〈0.05）, and SO2, Hb, K^＋ indexes were higher in male young rabbits than those of female ones（P〈0.05）, no sexual difference was shown in the blood gas of senile group（P〉0.05）. （2） Effect of age, the PCO2, BE-ECF, BE-B, SBC, HCO3, TCO2, a/A indexes were higher in young group than those of infant group （P〈0.05, P〈0.01）, while PO2 SO2, O2Ct, A, PO2/FI indexes were lower in young group than those of infant group （P〈0.05,P〈0.01）, pH, PCO2, BE-ECF, BE-B, SBC, HCOG TCO2, a/A, Osm indexes were higher in senile group than those of infant and young groups （P〈0.05, P〈0.01）, while PO2, SO2, Hct, Hb, O2Ct, A, PO2/FI indexes were lower in senile group than those of infant and young groups （P〈0.05, P〈0.01）. Conclusion Gender has effect on Glu in infant group and on SO2, Hb, K^＋ in young group. The blood gas, electrolytes and energy metabolism in WHBE rabbits were changed with increasing age, and those related with its physiological function.