改良吸烟复合气道内注入LPS法COPD大鼠模型的建立

目的对大鼠被动吸烟复合气道内注入脂多糖（lipopolysaccharide，LPs）法建立慢性阻塞性肺病（COPD）模型进行改良，通过比较模型大鼠肺功能、肺组织病理学改变、支气管肺泡灌洗液细胞分类计数及TNFα浓度、模型制作成功率和死亡率等指标评价改良烟熏复合气道内注入LPS法COPD大鼠模型的有效性。方法30只SD大鼠随机分为正常组、模型组和改良模型组三组，予正常组大鼠常规喂养，模型组采用第1及第15天气道内注入1g／ml LPS0.2ml（当天不行香烟熏染），第2-28天行每日3次（每次间歇2h），每次10支香烟熏染30min。改良模型组除调整第15天气道内注入LPS剂量为0.1ml，第2-7天每次香烟熏染剂量为5支外，其余同对照组。于第29天比较三组大鼠的肺功能、肺组织病理学改变及支气管肺泡灌洗液TNFα浓度、模型制作成功率。结果与正常组比较，模型组、改良模型组COPD模型大鼠气道阻力、支气管肺泡灌洗液TNF仪浓度明显高于正常组，两者肺顺应性明显低于正常组，结果有显著统计学差异（P〈0.05）。结论改良大鼠被动吸烟复合气道内注入LPS法是更为经济、稳定和可靠的大鼠COPD造模方法。

A Modified Passive Smoking and Endo-tracheal Instillation of LPS Method for Establishment of COPD Models in Rats

Objective To evaluate the efficacy of a modified method of passive smoking and endotracheal instillation of LPS for establishment of chronic obstructive pulmonary disease （COPD） model in rats. Methods 30 Sprague-Dawley（SD） rats were randomly divided into three groups; normal, model and modification groups, 10 rats each. The rats in normal group received normal feeding. The rats of model group were endotracheal instilled LPS （1g/L, 0.2 ml） on the first and 15^th day （no passive smoking on the same days） and they received passive smoking from the 2^nd day to 28^th day. The rats undertook passive smoking for 3 times. Each time they received 10 cigarettes lasting for 30 minutes and the interval time was 2 hours. Except the adjustment of the dose of LPS to 0.1 ml on the 15^th day, the dose of cigarettes to 5 from the 2^nd day to the 7^th day, Other performances in the modification groups are the same as those in the model group. On the 29^th day, the pulmonary function, histopathology changes, concentration of TNFα in bronchoalveolar fluid （BALF）, success rate and mortality rate were compared among three groups. Results Compared with the rats in normal group, the latter groups rats＇ airway resistance and concentration of TNFα in BALF were much higher. Conclusion This modified method of passive smoking and endotracheal instillation of LPS for establishment of COPD models in rats is more economic, reliable and worthy of application.