SPF级大鼠自发性乳腺肿瘤的流行病学与病理学特点

目的ICR小鼠感染近平滑念珠菌试验前注射环磷酰胺,观察环磷酰胺对近平滑念珠菌感染ICR小鼠白细胞的影响。方法尾静脉注射近平滑念珠菌感染动物前,连续腹腔注射环林酰胺3次,同时设环磷酰胺组、近平滑念珠菌组和生理盐水组。每天采集血样,对动物血液白细胞进行分类计数。结果近平滑念珠菌组:6～7d白细胞高于正常水平,8d恢复正常水平;环磷酰胺组:1～6d白细胞数低于正常水平(第4天最低),7～10d高于正常水平(第9天最高),11～13、15、20d白细胞恢复正常水平;环磷酰胺作用后近平滑念珠菌感染组:1～6d白细胞变化趋势同环磷酰胺组,在7～11d白细胞呈上升趋势,并显著高于正常值,动物全部死亡。结论环磷酰胺能够在一定的时间段能有效降低ICR小鼠白细胞数,导致免疫功能低下。在近平滑念珠菌感染小鼠实验中得以充分利用。

Epidemiologic and Pathologic Characterization of Spontaneous Breast Tumors in SPF Rats

Objective To investigate the effect of Cyclophosphamide on the white blood cell of C. parapsilosis infectted mouse. Methods ICR mouse were given cyclophosphamide once a day for 3 days before C.parapsilosis inoculation .The other mouse were injected with C.parapsilosis, cyclophos- phamide and N.S respectively as control groups. Then the mouse blood was collected and the blood were analysised. Results After C.parapsilosis inoculation, Cyclophosphamide treatment resulted in an imme- diate depression of peripheral blood leucocytes, with WBC and lymphocyte rebounding quickly to normal and rising throughtout the 2-week observation period, while mouse not treated with cyclophosphamide did not. And the cyclophosphamide model mouse, the number of WBC down quickly after inoculation , while up on the 5 day and recover to normal on the 11 day. Conclusion The WBC can be depressed effectively in some times with Cyclophosphamide; Cyclophosphamide can result in immunosuppression and play an important part in C.parapsilosis murine model.