五指山小型猪糖耐量试验及血脂分析

目的 确定五指山小型猪的正常糖耐量水平,并检测血脂,筛查糖、脂代谢异常小型猪.方法 选择未去势的五指山小型猪,禁食不禁水12h后按1.2 ml/kg体重的剂量静脉注射50％葡萄糖注射液,并分别在注糖前、注糖后10、30、60、90和120 min采血测定血糖值.同时采取空腹血清送实验室检测血糖（Glu）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）等生化指标.结果 静脉注糖后血糖水平出现显著性波动,注糖后10 min内血糖水平达到峰值,随后逐渐下降,直至注糖后90 min恢复到给糖前水平.有7头小型猪的糖耐量部分血糖值超过95％置信度上线值,有12头小型猪部分相关的血清生化指标值超过95％置信度上线值.相关性分析发现Glu与TG、TC、HDL-C、LDL-C这4个指标都存在显著的相关性,多因素分析表明TG、HDL-C与Glu显著相关.结论 筛选出糖代谢或脂代谢异常,以及糖耐量减低的小型猪.

Intravenous Glucose Tolerance Test and Analysis of Blood Fat in Wuzhishan Minipigs

Objective To screening the level of intravenous glucose tolerance test （IVGTT） and determine blood fat in Wuzhishan Minipigs （WZSP）. Methods The WZSPs without castrationwere injected i.v. 50% glucose injection by 1.2ml/kg after 12 hours with water but fasting. The blood-glucoses were tested by fast blood glucose meter at the time of fasting, and 10, 30, 60, 90, 120 minutes after glucose injection, respectively. Meanwhile, the fasting serum samples were taken for testing biochemical parameters such as GLU, TG, CHOL, HDL-CH, and LDL-CH. Results The level of blood-glucoses was significantly fluctuant after intravenous glucose in WZSP. The level of blood-glucoses reached peak value at 10 minutes after glucose injection, then dropped gradually, and recovered at the time of 90 minutes after injection. There are 7 minipigs whose partial blood-glucoses exceed the upper bound of 95% degree of confidence in the IVGTT. And the blood serum biochemical parameters of 12 minipigs exceed the upper bound. It was found that GLU is significantly relevant to TG, CHOL, HDL-CH and LDL-CH by correlation analysis. Multiple linear regression analysis shows that GLU is significantly correlative to TG and HDL-CH. Conclusion The minpigs were screened out, which were abnormal in glycometabolism, lipid metabolism, and impaired glucose tolerance. And the minpigs could be bred which are susceptive to type 2 diabetes.